Examples

Illustration of primer and gRNA design

Below, we present the command to run SMAP design and summary plots of twelve designs for different species and gene family sizes created with SMAP design to illustrate typically expected output.

The user is advised to run SMAP design first with the mandatory and default settings, and then decide on the most optimal parameter settings for your own design. The example data shown below are merely meant to illustrate the expected outcome of data sets processed with parameters adjusted to the specific species and reference (gene) sets. SMAP design parameter settings are described in the section Commands & options.

Command used to run SMAP design on a small gene family from Arabidopsis:

python3 SMAPdesign.py HOM04D000931_ath.fasta HOM04D000931_ath.gff -g HOM04D000931_ath_FlashFry.tsv -o HOM04D000931_ath_SMAPdesign -minl 120 -maxl 150 -smy -v -sf

Summary plot

Command used to run SMAP design on a medium-sized gene family from Arabidopsis:

python3 SMAPdesign.py HOM04D000029_ath.fasta HOM04D000029_ath.gff -g HOM04D000029_ath_FlashFry.tsv -o HOM04D000029_ath_SMAPdesign -minl 120 -maxl 150 -smy -v -sf

Summary plot

Command used to run SMAP design on a large gene family from Arabidopsis:

python3 SMAPdesign.py HOM04D000001_ath.fasta HOM04D000001_ath.gff -g HOM04D000001_ath_FlashFry.tsv -o HOM04D000001_ath_SMAPdesign -minl 120 -maxl 150 -smy -v -sf

Summary plot

Command used to run SMAP design on a small gene family from Chlamydomonas:

python3 SMAPdesign.py HOM04x5M006964_cre.fasta HOM04x5M006964_cre.gff -g HOM04x5M006964_cre_FlashFry.tsv -o HOM04x5M006964_cre_SMAPdesign -minl 220 -maxl 250 -smy -v -sf

Summary plot

Command used to run SMAP design on a medium-sized gene family from Chlamydomonas:

python3 SMAPdesign.py HOM04x5M000141_cre.fasta HOM04x5M000141_cre.gff -g HOM04x5M000141_cre_FlashFry.tsv -o HOM04x5M000141_cre_SMAPdesign -minl 220 -maxl 250 -smy -v -sf

Summary plot

Command used to run SMAP design on a large gene family from Chlamydomonas:

python3 SMAPdesign.py HOM04x5M000042_cre.fasta HOM04x5M000042_cre.gff -g HOM04x5M000042_cre_FlashFry.tsv -o HOM04x5M000042_cre_SMAPdesign -minl 220 -maxl 250 -smy -v -sf

Summary plot

Command used to run SMAP design on a small gene family from Soybean:

python3 SMAPdesign.py HOM04D000162_gma.fasta HOM04D000162_gma.gff -g HOM04D000162_gma_FlashFry.tsv -o HOM04D000162_gma_SMAPdesign -minl 400 -maxl 800 -d 150 -smy -v -sf

Summary plot

Command used to run SMAP design on a medium-sized gene family from Soybean:

python3 SMAPdesign.py HOM04D000015_gma.fasta HOM04D000015_gma.gff -g HOM04D000015_gma_FlashFry.tsv -o HOM04D000015_gma_SMAPdesign -minl 400 -maxl 800 -d 150 -smy -v -sf

Summary plot

Command used to run SMAP design on a large gene family from Soybean:

python3 SMAPdesign.py HOM04D000001_gma.fasta HOM04D000001_gma.gff -g HOM04D000001_gma_FlashFry.tsv -o HOM04D000001_gma_SMAPdesign -minl 400 -maxl 800 -d 150 -smy -v -sf

Summary plot

Command used to run SMAP design on a small gene family from the human genome:

python3 SMAPdesign.py HOM03P000828_hom.fasta HOM03P000828_hom.gff -g HOM03P000828_hom_FlashFry.tsv -o HOM03P000828_hom_SMAPdesign -minl 220 -maxl 250 -rpd -d 15 -smy -v -sf

Summary plot

Command used to run SMAP design on a medium-sized gene family from the human genome:

python3 SMAPdesign.py HOM03P000059_hom.fasta HOM03P000059_hom.gff -g HOM03P000059_hom_FlashFry.tsv -o HOM03P000059_hom_SMAPdesign -minl 220 -maxl 250 -rpd -d 15 -smy -v -sf

Summary plot

Command used to run SMAP design on a large gene family from the human genome:

python3 SMAPdesign.py HOM03P000013_hom.fasta HOM03P000013_hom.gff -g HOM03P000013_hom_FlashFry.tsv -o HOM03P000013_hom_SMAPdesign -minl 220 -maxl 250 -rpd -d 15 -smy -v -sf

Summary plot

Using the GFF file generated by SMAP design, a graphical view of the amplicons and gRNAs can be obtained with a genome browser software such as CLCbio Genomics Workbench, or Geneious.

In Geneious it would look like this.

../_images/SMAP_design_manual_overview_output_Geneious.png

The green arrows show the genes, the blue arrows show the amplicons with indication of the amplicon name, the dark and light green arrows show the forward and reverse primer, respectively, the grey arrows show the gRNAs, and the dark and light blue small arrows (both in the forward direction, and partially overlapping with the 3’ end of the primers) show the borders used for SMAP haplotype-window.

Option restrictedPrimerDesign

The --restrictedPrimerDesign or -rpd option restricts amplicon design to exonic regions and will ignore large intronic regions. This speeds up the primer design and can increase retention rates, because the 150 amplicons that Primer3 designs by default will no longer be located in intronic regions, and subsequently discared because there is no overlap with a CDS. Below are some examples comparing the design of three human gene families (with typically very large introns) with and without the -rpd option. The time needed to run these on a single core is given.

Without -rpd

Runtime: 00:00:26.69

command

python3 SMAPdesign.py HOM03P000828_hom.fasta HOM03P000828_hom.gff -g HOM03P000828_hom_FlashFry.tsv -o HOM03P000828_hom_SMAPdesign -minl 220 -maxl 250 -d 15 -smy -v -sf

With -rpd

Runtime: 00:00:26.56

command

python3 SMAPdesign.py HOM03P000828_hom.fasta HOM03P000828_hom.gff -g HOM03P000828_hom_FlashFry.tsv -o HOM03P000828_hom_SMAPdesign -rpd -minl 220 -maxl 250 -d 15 -smy -v -sf

Without -rpd

Runtime: 94:22:08.77

command

python3 SMAPdesign.py HOM03P000059_hom.fasta HOM03P000059_hom.gff -g HOM03P000059_hom_FlashFry.tsv -o HOM03P000059_hom_SMAPdesign -minl 220 -maxl 250 -d 15 -smy -v -sf

With -rpd

Runtime: 14:35:23.75

command

python3 SMAPdesign.py HOM03P000059_hom.fasta HOM03P000059_hom.gff -g HOM03P000059_hom_FlashFry.tsv -o HOM03P000059_hom_SMAPdesign -rpd -minl 220 -maxl 250 -d 15 -smy -v -sf

Without -rpd

Runtime: 56:06:46.92

command

python3 SMAPdesign.py HOM03P000013_hom.fasta HOM03P000013_hom.gff -g HOM03P000013_hom_FlashFry.tsv -o HOM03P000013_hom_SMAPdesign -minl 220 -maxl 250 -d 15 -smy -v -sf

With -rpd

Runtime: 08:57:11.93

command

python3 SMAPdesign.py HOM03P000013_hom.fasta HOM03P000013_hom.gff -g HOM03P000013_hom_FlashFry.tsv -o HOM03P000013_hom_SMAPdesign -rpd -minl 220 -maxl 250 -d 15 -smy -v -sf

Option preSelectedPrimers

If a set of (validated) amplicons is already available and you want to find gRNAs within these amplicons, this can be done using the --preSelectedPrimers or -psp option. If this option is used, primer3 will not design any amplicons, but will merge the existing amplicons with the gRNAs to generate the designs. The user should provide a GFF file containing the alternating features: Primer_forward and Primer_reverse, whereby the Primer_reverse forms a pair with the Primer_forward just above it. Any other options concerning the amplicons are ignored, except the –ampliconLabel option.

Examples of files required as input using option --preSelectedPrimers.

Reference sequence FASTA

>AT1G49160

GCGACGACCCGTGCCATGCAGGTTGAAGTCACTTTCAATGATCGTTGTCTCATAAATTACAAAGAACACAGCTATTTTGTTTCTATTTCATAATGAAAAGCGAATATTTGTCTGTTCAGATACCGATCAGGTAAGCGATGCTTAAAGCCACCAAAAGGGATTCTAAAGTAATCAATGATTTGATTAAGAAAAAAGTCAGAAAACAATATCCATTTGAGTATTTCCATGTCACCAATTGGTTTATAACTTCCAATGATCACATGCCGCTTGTAATATACATCACCTAACCAAGCGATCGACTCCAAAAACGAAACCGTATTTATTATTATCTTAAGACTATAGTCTATACAATATACATTACATAATCAAGATGCCCAATTAGATGTTTTAAAACAACAAAAAGATCAATAGGCCCAATTATATCTTATCTTAATAAAATGAAAAAGCAATTGCATGAGGAATTATGGATATATATAAATTACATGGGCTAGGTTTTTTTTATATACATATAAATAGTTTGGAGAGATAAATCAGGTGGAACCAAGTTTGTTGTTTGTGTTTCGATTGAACAACACTTTAATTATTTAATCTTTCCATTGTCTTTGCGTCTTCTTCGATCAATTTATCAAATTAGGGTTCCAACCTTTTTTCTTTTTTTTCCCCGTTGTGTGTTAAGTCGGCGGAAAAACTTAGAACAGAACAAACCATCATTTTTCCAGGTAAATTATATTCTAATCTCATGTTCTTGTTAATATCTGATTTTTGATGATCTTCCTATGCATGTTTGTCATTGCGTCTTTCTCTTACGTGATAGATGTTCGTTGACGCGATTTGAGGTTTATTTAGGTTCCTTAATAGATTTAACAAAAAGAAATTCTCGGTCAATAATATTTATTTGAGCTGATCAATCATTTGCTGGAGTTAGTTTGTGTTGTCGTCTTCATATGAATCTAAAGGGTATATATATGTTTTGCATTCTTTGTTATAGAAATTTGAAGAATAGATAAAAAAAGATTAATAAGATATGGAAGGTTCAGAAGATGCTTCTGCAATTGTTGAACCACCTGACCCAGAAGTTCTTGAAATCGACCCAACTTGTCGATATATTCGGGTAACTACTTCACCTTTATCTCTTCCATAATTGTTTTAATTCTTTGTTACCAAATATTAGGCTCGACCAAATATGCCCTGTTTCTCCATTTTGTTTTCCCTTGACACATTCAAATGTGTAAACCTTTCAGTACAAAGAAGTAATAGGCAAAGGCGCATCCAAGACAGTGTATCCCTTTCTCTGTTTTTCTTTACATAATCTCTGTTTTTGTTTTACAATCTGTTATTCTGTGTTATGTGACATCATAGTTTGTGCTACGAGAGGATATGAAATTCATTATTTCTTTAACTCTCTTGAACAAATAATCAGTTTTAAGGGATTCGACGAAGTAGATGGGATTGAAGTAGCGTGGAATCAAGTAAGGATCGACGATCTTTTGCAATCACCAGATTGTCTAGAGAGGCTATATTCCGAAGTGCGGCTTTTGAAATCTCTGAAGCATAAAAACATCATAAGGTTTTATAACTCGTGGATCGATGACAAGAACAAGACGGTTAACATAATTACCGAGTTGTTCACTTCAGGAAGTCTCCGGCAGTATGTATTTTGCTCATTTTTATACTATCTTGTTATGTCGCCGCGAAAATAGCGGTTTTGGAGTTGTGTTTAAAGCAGCGAAATCTTGGTTTTCTTGAAGGTACCGTAAGAAACATAGAAAGGTGAATATGAAGGCTGTCAAGTGTTGGGCGAGACAGATTTTAACGGGATTGAAATATCTACATAGTCAAGATCCGCCGATAATACACCGAGATATCAAATGTGACAACATTTTTATCAACGGTAATCATGGAGAAGTGAAAATAGGAGATCTTGGTTTAGCTACTGTCATGGAACAAGCTAATGCCAAAAGTGTGATTGGTATGAGCAATGCTTTTTCTTTTATATAGTTTACAAATTCGTTTTTCTTTGTTTTTTTTAGGATCTAAATTGATAGTGTTATGTTATAACCAACAGGAACACCGGAGTTTATGGCGCCTGAGCTTTACGATGAGAACTACAACGAGCTCGCTGATATATATTCGTTCGGGATGTGTATGTTGGAAATGGTGACTTTTGAATATCCTTATTGTGAGTGTAGAAACTCTGCTCAAATCTACAAGAAAGTTTCATCGGTAAGTTCGATATTTTCAAATTGTTTCTACCATTGGTTTTATGATATCGAACAATATATGATAATATGAATGGATAACTTACTTGGTGAGTCTTTTTTTTGTATTCTTTACTTAGGGGATCAAACCAGCTTCACTATCAAAGGTTAAAGATCCAGAGGTAATGAAATTTATCGAGAAATGTTTATTGCCAGCTTCCGAAAGGTTATCGGCAGAGGAGCTTTTATTGGATTCTTTTCTCAATGTGAACGGTTTAGTTATGAACAATCCATTACCGCTTCCCGACATTGTAATGCCGAAAGAAGGATCATTCGGTGAACGTTGTCTTATGTCTGAAGGACCACCTAATGCTCGGAATAGAACGATGTCAATGAATCTTGACGAAGACAATAACCTTCCTATTGTTATTTCAAGCAACAATTCAGGAACAAACTGTATTGAGGTTAGACGGGCAAAGCGAGGAAACTTTTTTGTCCTTAAAGGTGAAGAGAATGATGAGAACTCTGTCTCATTAATTCTTCGTATAGTCGATGAAAATGGTAAGAGTCTTTTGCGTTTATATTTTACAAAACATTTCACCGAAAATGTTAGATGCATATACAATCAGCCAAAAATTGCGTTTGGAAAATTGCATATTATACCAAACTTGATTAGTTTTGTCTTCTTTTTGGCGTGTAGGGCGTGTGAGGAACATTCATTTTCTATTCTTTCAAGAAGGTGACACTGCTTCTAATGTATCTAGTGAGATGGTTGAACAGCTTGAATTGACGGATAAAAACGTAAAGTTTATAGCGGAATTGATTGACGTGTTACTCGTCAATTTGATACCGAATTGGAAAACCGATGTAGCTGTCGATCATCTAATTCATCCGCAACAGAACCAGAGCTCCAAGGATAATCATCAAAACGGTGCAAGTTCTCAAGCTGGTGAATCCATTAGCCACTCTTTGTCCTCTGATTATTGCCCACGATCCGATGATGAGGCAAACCCGACCGTAGCTGCAACAACAGAAGATCAAGAAGCAGAGAAACCAGGAAGCTTGGAGGAAGAAGAAGAAGATGAGAGGTTAAAAGAGGAGTTAGAAAAGATAGAAGAACGGTTCAGAGAAGAGATGAAAGAGATAACGAGGAAAAGAGAAGAAGCAACAATGGAGACCAAAAATAGATTTTTCGAGAAGAAGATGCAACAAGTTGAGTAAAGTAAACAACAGAGCAACACTCTGGTTTTGGATTCCTCTGTTTTGCTTTTCTGTTTTTCCATGTTTTATTATTTTAGTTTTGGTCAAAGAGAAAAAAGCTTTTGTTGTTTACTTGTTTCATTGCAAGTGTAGAAAGTTTCCTCCATAAAAAAAATAGAAAGTATTTTCTAATGGTAATTGGGATTTACATTATATAAAGCACTAATTGCTGAGATAAAACCATTCATCAGGTATATATGTAAAACTATTCTCGAGATAAAACAAGAAAGTGAATACACAATTTTCTTGCCTCTATTCATAAGAAATAAACCTGTTTTCATTGTGTTTATTTAACACCAAAAGCACTCAAAATTGAGGCTTAACATTCAGACAAACCGTAACTATAGAACATAAGAAAAACCTAACTATTGAAGAGGTTGCATTATATCACAAGTTTAAAACACCAAAGCTCAAGCTGAGTAGCAAAAATAGGTCTCTTCTTCCGCCACCAAAACCTCAACAGGAACGTCTACAAAAGCCTTGACCGCCACAACGACCACCGGAAAAACGACCACCGACGAAAAGACCACGACCACCACCAGGACCAAAGACAGGAGTACCAAAACGGCTATAAGGTTCCTCATCAGAAGATGATCCATCCACAGACATGCTCTCTGCCTGGGCTTTACAGAGCCAATTTTAACTTCATTATTTGACCTAAACAATGAGATCAACAGATCC

>AT1G64630

TTCTTGGAGATAGGACAAGTGGTGAGAGGATTGGGATTGAAGATATTGAAAGGAGTGATGGAAACAAGAAAAGGACAAATATGGGCACACTTCATCGTACAGGCAAAGCCACAAGTCACAAGGATTCAAGTCTTGTACTCACTTGTTCAGCTCTTTCAACACCACACCAAACATGATGATTTATTATCATAGTATAATACCTACCACTTTGATTTTCTTTCTTTTTGTTACAACAACAACACAAGAATCTGTTGATAAATAGTAGTAACATTACATCAAAGAAGATTGTATGGGCTCGTGGAAAAAAAAGAAGAAGATTGTATGGGCTTTAAAACATTGACTAAGACAAAGAAAGAAGGCCCATTTAAAGAAAAAAGGAACGTAACGACACCGTTTTGGGTGGATAACAGTTAACTGACTGAATCAGAAAATAGATGCCCGTTATCCTTGACGAGGATAAAGGGGAGACACTGTATATAAATAAAAAGTTGAACAAAGATCAAATCCTCAATCTGTTTTTAGGGTTTCGAGAGAGAGAGAGAAATTGATTTTTGTTGATATTTGTGAAATTTGGTGGCGAAATTGAAGCTTCTTCATCGGTCGTTGTCTAAAAGCAATGGTGGATGGTTGATGAATGGAGGAGATGCTCTTCTTCTTCTATCCAATAACAATAATAAGAGACACGACGCTGACACAAACGCAGAACCATCTCACCACCGTCATCATCTCGGAGATGGCCGCCACACTCCTCTGTTCCCGATCTCGATTCCGATCGGCGATCGACTCCTATTGCTTCGCCCGTGAGGCCTTCTCCGATCCAATCTCCTTCCATCTCCTCACCCTTCTGTAACCACCAATCATCATCCCTCCTTTCTTCTTCTTCTTCTTCTGCTTCCGGATTATTAGTATCATTAGGTTAGAATATCTGCCATGGAAGAGGCTGACTTCGTCCAGAAAGATCCCACTGGTCGCTACATTAGGGTACGACGCTATCTGCAATTTCCATGGTTTCTAGGATAACAATGTCAATCTATAGACTTTTCCTCGATAAGATTTATATAAGTAATAGCTACGTTAATGGCTTGGAAGGTCACTTTAGGATAACAATCTTCTTCTTTCTTTCTTCAGTATAATGACGTTCTCGGGAGAGGAGCCTTCAAAACTGTGTATGTTTGTTTGTTTATACGTCCTTTACTTTTTCATTGCAGTGCCTATGTTACTCTGATTGCTTTTTAAGTATTTTTCTCATCTCTTGTTGTTGTTGTTACCTCGCCGCGTGTAGATATAAGGCATTTGATGAAGTCGAAGGTATTGAAGTTGCTTGGAACCTCATGAGCATTGAAGATGTCTTGCAGATGCCTGGCCAACTTGATAGGCTATATTCCGAAGTCCATCTCCTCAATTCCCTTAAACATGATAACATCATCAAACTCTTCTACTCTTGGGTTGATGATCATAACAAGTCCATCAACATGATCACTGAGCTTTTCACCTCTGGTAGTCTCACCCTGTATGTCTACTTACTCCTCTCCTCTCTTCTTCTCTTTTCATTATCTCTTATAATATATCTACGTCTGCCTTTCCATTGATTGTCATTTCTCTTGGTCCAAAGTTATCGCAAGAAGCACCGCAAAGTTGATCCCAAGGCCATCATGAACTGGGCAAGGCAGATTCTTAAAGGCCTGCACTATTTGCATTCTCAGACCCCTCCTGTAATTCACCGTGACCTTAAGTGCGACAATATTTTTGTCAATGGAAATACCGGAAAGGTCAAAATTGGAGATCTTGGGCTCGCTGCTGTGATGCAGCAACCCACTGCTCGAAGTGTGATTGGTAATAATACTATTAAAACCTCTTTAAGTCTTGTTGGTTTTAGGATAAGCTTTTCTAGAACTGAATATATTGAGTATGTTTTTCAGGCACTCCTGAATTCATGGCGCCTGAGCTTTATGAAGAAGAATATAATGAACTTGTCGACATCTATTCTTTTGGCATGTGCATGTTGGAGATGGTAACATGTGAATATCCATACAGAGAATGCAGAAACCAGGCTCAAATATACAAGAAGGTTACCTCGGTAAGTCTGACCTGATATATATAAGTCTTACATTTCTCTTCGACACAGGGATCGTCCTTGACCATTGTTGCATTAGCAGGGTATAAAACCTCAATCTCTCAGCAAAGTTGATGATCCTCAAGTTAAGCAATTCATAGAGAAGTGTCTTCTCCCAGCTCCTTCTAGACCAACTGCTCTAGAGCTCCTGAAGGACCAACTCCTTGCAGTAGATGGCGCCAAGGACTCCACTCTTACTGCTTCATCAAACACAACATTCAAACCTGCAATGCCACCACAGTGTGAATATCGTCCTATGGATGTTGAATATAAGAAGAATACAAGTGTTTCCATCTGCTCTTCTGCTAAAAGCAGCCAAGAATGCGCATTGCTCCAGACCATGGAAGTCCAGAGGGTTGCTGAAAGTACCGAATTCAAGCTGAGTGGAGAGAGGAGAGATGATGTGGCAGCATCAATGGCTCTGCGGATCGCGGGCTCATCTGGTCTGTTTCACGATATCTTATGGCCATTTTCTTTTTCTCTTCTCTTGCATATTTTCGAATTGCTTAATGACAGTATAAATTTTATATCAGGTCAAGCGAGAAAAGTTGATTTTGACTTCAATCTGAAGACAGACACAGCAAGAGCAGTTACAGGGGAGATGGTTGAAGAGCTTGATCTGTCAAGCCATGAGGTTACTGTGATAGCTGAGATGATAGATGAACTGATAATGAAGCTGAAGGCTAATCGAAGCCTGCCTAATGCAAACAGTGTATATCAGTCCAAGGATGAAGAAGCTGGAGAATCAATGAAGTCAGAGATATCAGCGGACTACTACCATCGGGTCTCCTCAAACGAGGGGTCACGGCTCGGCTGCTGCTGCGAGGCAGTAGAGTCATTGTTGTCCTCGTTTCTGGATTCATGCTCAATGGTGTCAAACAAGCAGTCGGAGGATCTGAAGACGGAGCTGAATGTGATAGAGTCACAATATAACCAATCGTGTCAACGACTGCTTAGAATGAAAGAGGAAGCTATAGAGAAGGCGAAACGGAAGTGGATGAAGCTGAGTTGATGATTCCTTCATTTCATTTAACGTAGCCACAGTTTTGGGATTTACTCAACTTTTTGTTTTGTTTCTTTGCCTTTTATTTCATTTCTCGTAGCTTTTGGTTACATGTAAAAATATGCATTGCATCAGGAATCACAGATTGAAAGAATGAAATGTATTTGACGCTAATTCAAAATTTAGAACTATATGGATTGTGTCATTGTGTGTACATATCTGTTAATAGTTTAGAAAATTGGCAAATTCATTTTCGAGATATAAAAAGATAGGAGCCAATGAAGAAGAATAAATAATTAAGAGATGATAACAAAAACAAAACAAAAAAGAAGCAATGACTGTTTGCTTTAATAAATAAAGATATTCAAAAATTCCATCACATGATTGCACCGACGACAGCGCAAGCCAGCGAAGCAGCGACAGTGGAGAAAGCAGAAATGAGCGAGGAGGAAGCAGACGATTTCTGAGAGAGAGGAATGGACCCGAAGGCGTTTTGGTAAGAGGGAGCGTCGGCGGCAGAAGACGGAGACAAGGCCTCTGCTTCGGCGGAGTAGGTGCCGACGGAGACTAGGAGCTTCTGGTACTTGGTACAGTGGCCGGGGTTTGCACTTGTGAAGTATAGGGTTCCGTTTTGGGTGAGGTTGAAGAGAGAGTTGCCGTCGTTCATGTACAAGATTGGGTCTTTGGTGTTGCAGCTCTTGAAAT

>AT3G04910

ATATAATTTGTTTTGAATGTTGATTGTTCTAATTGAAATGAAAACCAGTCGAAGTTTTTAATGACATAAACTAAACCTTTATCAGAATATGGTTCTAAATGTAGTAATTTGTTAATCATATCACACAGTTTAGCCGGTAGGGAGAATTTTTTTTCGGCACGTTATCTCGATGAGATTCATTATTTTGGTAGGTTCAACATTTATTTCACAACAATACTCCGTCGGCTAATGGAATTATTACTATTTGCAAGCATCATCTTTGTGTATAACAAAACAAAACTAAAATCAAAACAAAAGAAAGGTAAAGTAATAGTAACATTTTTGTTTTCTCTAGCTTGTGAATTTGTATAGAATAATCACTAAAATGAATCAATCATAATTTTCAAGTATTCAAGTTACTTTTCCACCGAACAATTGTTAATACTACCACAGAATAATTTCCAATAAATAATTGTCGATAGAGGACACTAATTATATATACCGTATAAAATAAAATCCGTTGCAATTATTTAGAAGTATGTAAAAAGGTTTAAGGAACAAAAAAAAGGTTCCATAAGAAGAAGAAGAAAAAAAATGAAGGATAGATATTTGTTTAGTTTATATCCCATTTTTGGACCACCGTTCCCTAAAGAAACGCACACCGTCTTTGTCTTCTCTCACGAGGTGCTTGTATTTCTCTGTCATCAACCAAAAAAAAAAAACTTCCATTGAACTATTGAAGAGAGAAAAGCTTGGCTTCTCTGTCTTCTAAGCTCAAATCCTCCTTTTCTCTCTCTCTCTATAGAGTTTGAGTATTTATTAGAATGTAATAATGAGAGAGTCTATACAGATACTGTTCTTATCTACTACATGAGTTTCATTGCCCTGTTCAACTCCTTTTGTCAGAGAAGAACAAAAACAATAATATGATTATGCAGAAATTGCTATAAGAAGAAACCTAAACTCTCTCCCATTTATTAAAAGAGAGACGTTTGTATTGGTAGATCTATTCTTATTTTTCCTTTTGGGATTTTTTTATTTGGTTCGTAATTGTGAGATTGAAAGAGAGATGAACAATCTGAGTTATCTTGAACCAGATTACTCTGAGTTTGTTGAAGTTGATCCTACTGGAAGATATGGAAGAGTAATGTTTCCTCCTATCAATCAATCAATCTCAATTATTTTGTTTGATCTTTTGTTGACTTCATTGTTCTTGTTGTTGCAGTACAATGAAGTTCTTGGTAAAGGAGCTTCAAAGACTGTGTGAGAATCTCTCCCTTCTTCTCTTCTTTTTCTGCTTCATGTTTCTCTCAAAGTTTGCAGCTTTTTTTTCTTCTGGATCTGGTGATGATTTAAGCCTAATCTGTTGTTCTTTCTTCATAAAGTTTGTAACTTTTTGTTTTCCCAGTCAAAACCCATTTGTGATCCAACAAATTTGTCCTTACGATTTTTGTGTGTTTTGGATTCATGATTGAATCAAATCCTTTAAAAGAGGAAATAAAAAAGTTGAGCTTTTGTTTTTTTTTGCTTCATTGAATCACTTCTAGTTTGGTCATTAATTCCCCACAAGAGAAATTTCCAACAACTTTGCTTTTTTTGGATCTCTTTATTGCTTTGACTTATACCAAATTCAGATTCTGCAAACAAAAAAACTTCTTTGCTCATACATACATTAATCTTCTTTCTTTCTTTTGATTTTGTAGTTATAGAGCATTTGATGAATATGAAGGCATAGAAGTAGCATGGAACCAAGTTAAGCTATATGATTTCCTACAAAGCCCTGAGGATCTTGAGAGGCTTTACTGTGAGATTCATCTTCTCAAGACTCTAAAACATAAGAACATCATGAAATTCTACACTTCTTGGGTCGATACCGCCAATCGAAATATCAATTTCGTCACTGAATTGTTCACTTCTGGCACCTTAAGACAGTAAGATTCTCTTGTAAACATACTTTTCTGTCGTTGGTTTTAGTTTATCTTTAACGATAACTTCATTTATGGATACAGATATAGACTCAGACATAAGAGAGTGAACATAAGAGCTATGAAGCATTGGTGCAGACAAATCTTGAGAGGCTTACATTATCTTCACAGCCATGATCCTCCTGTGATCCACAGGGATCTCAAATGTGACAACATTTTCGTTAACGGGAATCAAGGAGAGGTCAAGATTGGAGATCTTGGCCTTGCTGCTATTTTAAGAAAGTCCCATGCTGCTCACTGCGTCGGTACTATATGCAAATCCAAATCATATCCTTTCTGCTTTTTAGCTGAGTTTCTTTGTTTCTGATTGTGTAAAGTTTTGATCTTTTGTTAGGGACTCCTGAGTTCATGGCACCTGAAGTTTACGAAGAAGCATATAACGAATTGGTTGATATATACTCGTTCGGTATGTGTATTTTGGAGATGGTTACGTTTGATTACCCGTACAGTGAGTGTACTCATCCTGCTCAGATCTACAAGAAAGTTATGTCGGTGAGACTCTCCGATTACTAAAACACTAAGATAAAAAAGCTTCATCTCTTTCTGTTTCAAAAGGCTAAAAGTTCGGTTTCAGGGCAAGAAACCGGATGCATTGTACAAGGTGAAAGACCCCGAGGTTAAATGTTTCATTGAGAAATGCTTGGCCACCGTATCGCTTAGAGTCTCTGCTCGTGAGTTACTAGATGACCCTTTTCTCCGTATAGACGATGGTGAGTTTGATTTAAGATCAGTTGATATGGAAGATTCGGTCGGGCCACTCTATAGGCAGCCGCACCATCTTCCTGACTACTACAATTACCCGTCGAATAGTAGCTCTTTGAATCGTCAGTACTCAAATGGCAACTACCCGTCGAATAGTAGCTCATTGAATCGTCAGTACTCAAATGGTTATAATAGTCATCACGAGTATCAGAATGGATGGGCGTATAATCCAGCTGAGACAGAGGAGACTCACGGCATCGAGCTCTTTGAATCTCGAAACAATGATGATCAAGAAGAAGAAAAGAAATCTGGTAATGTTGACATAACCATCAAAGGGAAGAGGAGAGATGATGGTGGCTTGTTCTTGCGTCTTAGGATCGCTGACAAAGAAGGTAACAATATTCTCTTTATCTTTTCCTTGTTTCATAACCTATGAAGAAGAGTGGAAAAAATGCAGTTTCTTTCATTTGATTCATGGACTGGTTTGTTGCAGGACGTGTCCGAAACATTTACTTCCCATTTGACATTGAGACGGACACCGCATTGAGCGTTGCAACAGAGATGGTAGCGGAACTGGATATGGACGATCATGGAGTCACAAAAATAGCCAACATGATTGACGGTGAGATATCTTCTCTTGTACCTAGTTGGAGACCGGGACCTGAATTCGAAGAATGTCTTGCGGCTGCGGCGGCGGCAAATGCTGCGAGCATTTGCAACAACTGCGTATCAAACCGCACCTCAATGGGCTCGGTGATGGATTTCCTGAGAACCAATCCTGGGGCAAATGTGATACAATGTTGCAGAAACGGGTGCGGTGAGACTCATGGTCGGTTTGAAGAGATCACGATCAGAGAAACCGAGGTTCGTCTTAGAGAGCTATGGAAGCTGCAGCAACAGCAAGAAAGCCGCGAGCTAAGCTCGATAGATTCAGGCCATAACCATTCCGAAGAAGAGGAGGAAGAAGAGGTGCTATACGAAGACCCCGAAAACATGTTTTCTTGCGAGGCAGGTAACGAGATAAACCATATATCGGGTTCTGGATCGTTCTCGTTTATGCCATCTAAATACTGCGATGAGCCATCCGAAAAAACCGAAAATCAGGTCCAACAAGAGTTGAGATGGCTTAAAGCCAAATGCCAAATTGAGCTTAGAGATATTCAGGATGAACAACTAAAAACCCGGTGGCCGGAATCCGGAGAAGAGGTGGAAATTTCTCCGAAAGACGGGTTCTTGGGTTCGGTTTCCGGGTTAGGAAGAGAAGAAGATACGGTGAAAGAGATGTTTGGAGAAAGATTGGTACCAAAGTGTCTGAAAAGAACAACTTCACTTCCTGTTGATGCCATTGATTCTTGATTACATTCTGTTTCTTCATGTATACATACATATTGTTTCCTATTATAAAGACTTTTACAGTGTAACAAGAACAACACACAAGTATACTTAAGAGATCCTCTTCAAATTCTTTTGGGGTTATTTGTACAATAATACCCTTCAATCTAATAACTTCAAAATGCAATACGATTATAATTTTTTTTATTACTGATTACTTGAAAATTTTCGTTTCTAGCAAGTTGTTTTAAAATCTTTTCAGCATTGACATACTATAGCAAATGATAATAATTGATGACAAGCAATAATGATAGCAAATTATTGAGCGATGTGAAGTCAGCAATCAGTTTCGGAATAAAACTTCGGACAATAAAATTACTCAAAAGTACATTAATAACTTTAATAAAGAAAAACCAAAAAAAACAAATGAATTAGTGTCTACTATGTTCTTAAAACACTTTGGATAATGCAAAGAGAAATCGAATGAGAAAAATAAAAAAGGAAAAAAGTGACAAAAATAGTCAATTATAAGAGTAACCCCACATCAGCACAACGCCATGTTCCATTGCCGACCAATCAACTAGAAATTTGGGTTCATCCACATGCCTTTGTATTTATTTTTCATCAAAAATATGAGATATATATTGTATTAAATATAACATGGTTGGTTTGTCGTTACTATGTGATTATTATAGGAAATGG

>AT3G18750

ACAGACTCACTAGTGGGAGCTTATATGATGGATCCTCCTCGGACGAAGATGACAACAACATTTTAGGGTTATCACGATGAGGAAATCAATTTCTTTGAAATGTTTACTCAGACAATTTCTTCATGGGAGTCCCCTTCAATGGTTATGAATGTTTTTGGTTACATGAAGTGGTGGTATGCGTTTTGCGTAGACAATGAAATAAATTAATTTTATGTAATAATAATTTTTAGGTAAATTGAACTAAATTTCATGTGTTTTATTGTTTTCGCTATTTTCCTAGTGGATATTCAACGTCATCAATTGGGTTGACAACCCCGAATTAAATGCCACGTGTATTGACACGTCATATTTGCATGGGGAGAGCGTTTCTCTGTTTTTAGGACTTGGAAAAATTATCATTTCTAAAAATTAATATTGGGAAAAAAGAAGGAAATTAAAATAGAGGTGGTGGTTCGTAAATGAGCTAAGAACAAACACTTGTTATAATTTCCCGTGTCGCTGCATCTTCTTCTTCATCCATCGATTTCTCTCAATTAGGGTTCTGATTTTTCTTCCCATTGTTGTTCTCCTTCTCCGTTTCGTCTCAATTACAGAACAAAGCTTCGTTTCTCCAGGTATAATCGAAACGTCAGAATCTTATTAATACAAATTTACGCGATTCTGTTGCCTTATACCGTTTTTTTTTTTGAGAGAGAAAATCTTTTAATCGTGTGTTTTCTATGTTCATATGAATGTATGCATTGATGTGAATGTATATGAATGTATACTCATGTATGCATTTTTTTTTTCTCCTCTCAAAATTATGAAATAGTTCATGTCAATTGTTTATACCTGTGTCGAATGTGGATATTTTGACAGAATATAATCTGAGAAGAAGAGTATTGGATTGTTTTTTATAAAAATTATGTCACGATCCTAAATTTTGACGAGCTTTACTTTAATTTTGTTTTTGTTCTGTTTTGTCTTTGTTGTTGATATGAGCTGGATAGTAATCGAAGGTTAAAAAGTTTCACAAAAAATTGGATTTAGAGTTGAATTAGCGTGTGAGTATCTTGTTGTCCTTGAAATTCTTTTTATTTCAAGGTGTTGCACCCAAAAGAATCTAAAGGGTTTCTATGATCGTTCTGTGTTTTTATGGATTTGTTTTCTGTTGTCAACTTTTGCAAGGTTTAAGAAAGAATGGAAGGTACAGATGATGCTTCTGCACTTCAAGAGCCTCCTGACCCCGAAGTGCTCGAAGTTGATCCAACTTTTCGATATATACGGGTATGAAGAGGCTCTTTGGAAATTGGAATGCTATCTTTTTTCTATTTGTTAAGTGTTTTACCATTCAATTGGTCGATACTATGAAACCAAGTTCCACCTCCCTTTGGCTTGTTTTACTTCTTAATGTTAAGGTTTGAGTAAATATTGTGAGGTGATTTAGATCTCTGATGTTCTTAACCAATCAATTGTGGACCTTTCAGTATAAAGAGGTCATTGGAAAAGGGGCATTCAAGACTGTGTATCCTTTTCGGTTTGTTTTCATATTTATTTCGGAGATTTAGAGTATTCTTGATATCAGAAGCTTCCTAGAAATGTACTCATATCATTTTAGTGATTTTGTTTTTCGAAACTCTTTTACGAGAATGAATTAATATCTTTCTCCTATATTCTGCATCTTTTTTGGTTTGTACCTTAACTCAAGGAAATCAGTTATAAGGCATTTGATGAAGTAGATGGAATTGAAGTCGCATGGAACCAAGTACGGATAGATGATGTTTTGCAGTCACCGAATTGCCTGGAGAGGCTCTATTCTGAAGTGCGTCTGTTGAAATCATTGAAGCACAATAATATTATTAGGTTCTATAATTCATGGATCGATGATAAGAACAAGACTGTTAACATAATAACTGAGCTATTCACTTCAGGGAGCCTCCGGCAGTATGTATCTCTCTTCTTTTTTTTTTCTTTCCCTTTCTTCTTATTGAGGAGATCACTGTTATGGAGTTTAAGAAATCTTTGGTTTCAGCTACCGCAAGAAACACAGGAAGGTGAACATGAAGGCCGTCAAGAATTGGGCGAGGCAGATTTTAATGGGTTTGAGATATCTTCACGGTCAAGAGCCACCAATTATACATCGGGATCTCAAATGCGACAACATTTTTATCAATGGAAACCATGGAGAAGTGAAAATAGGAGATCTTGGTCTAGCCACTGTCATGGAGCAAGCTAATGCCAAAAGTGTGATTGGTATAGTTTCTATTTCCCGCTTGTTGGTGTTACTTACATATTCACTTTTTCTGGTTTAATCCAAGAAGACCGGATACAGTTGTAGCCATGCTATATTCTTTGTCTGTCACATTTGAATTTCTCTTCTTTTGGGGTCAAATTGTATGTTGCCAACAGGAACCCCGGAGTTTATGGCACCCGAGCTTTACGACGAGAACTACAACGAATTGGCTGATATTTATTCATTTGGGATGTGTATGTTGGAGATGGTGACATTTGATTATCCCTACTGTGAATGCAAAAACTCTGCTCAGATATACAAGAAGGTTTCATCGGTGAGTTAGCAACAAAGACATATATGCCACATAATCCCTGTCAATAGTTTACGGACAAGATTTTAACCGATTCTTCTTCTTGGTGATTTCAAGGGAATAAAACCTGCTTCACTCTCCAGGGTTAAAGACCCAGAAGTAAAACAGTTTATTGAGAAATGCTTACTTCCTGCATCCGAAAGGCTGTCAGCAAAAGAGCTTTTATTGGACCCTTTTCTGCAACTAAATGGTTTAACAATGAACAATCCTTTGCCGCTTCCTGACATTGTAATGCCCAAAGAAGGCGCATTTGGAGACCGTTGCCTTATGTCTGAAGGTCCTCCTACAACACGGCCTAGTAAAACCTTGTCGATAGATCTTGATGAAGACAGTAACCTGCCTATTGTTACTTTTAGCGACAATTCAGGATCCAGGTGTATTGAGGTTAGACGCGCAAAGAGAGGGAATTTCTTTGTCCTCAAGGGTGAAGAAAACGATGAGCAGTCTGTGTCCTTAATTCTTCGTATAGTAGATGAGAATGGTCAGTTCTTTATTCTGCTCCAGCTAAGATTCATTACTTTCTTCGATATTTTTGCATTCTGAAACTTAATATAACTGTGTCTTGTTGCACAGGGCGTGTGAGGAACATTCATTTCCTGTTCTATCAAGAAGGGGACACTGCTTCTAAGGTGTCGAGTGAGATGGTTGAGCAACTCGAGTTAACTGACCAGAACGTCACGTTTATAGCCGAGCTGATTGATATACTACTTGTCAACATGATACCCACCTGGAAAACCGATGTCACAGTCGATCATCTCATCCATTCGCAACTGAATCAGAACTCAAGAAGTCATCATAACGAAGCAAAGCCACAGAAACAAGAGGAGACTGTTTTTCATGATACTTGTGAGTTGGTTAGTCACTCGTGTAACTCAGATTGCCCACGATCAGATGAGGAAGATAAGCAATGCGTCGATGCCACCAAAGGAGAAGACAAGAGCTCCATTCAAGAAGTAGAAGAAGCAACCGAACCAGTAAGTTTGGAGGAAGAAGAAAGGTTAAGACAAGAGCTGGAGGAGATAGAAGCTAAGTATCAGGAAGATATGAAAGAGATAGCAACGAAAAGAGAAGAGGCCATTATGGAGACGAAGAAAAAGTTGTCTCTGATGAAGTTAAAGTAATAGCCAAAAAAGCTCAAAGAAAACGTTGATACTGATGAAGAGCTTTTGTGTTTTTAATCTCTTTTGTTTAATTTGTTGGTTGGAGGAGAAGTGTAGAAAGATGAAGGGTTTCTATTTGATTAATTGAGATTTAATTTGGTTGGTTGTTACAAGTTAGAACATAAAAAATGGTTCCTGTTAAAATGTTCTAAGAGATTGTCCATTATATATGATTTTGTATAAATTGAACATGTAATTAGTTAATAGCCAACTATTGTAATAAAAGTAATCAAGCCTTTTCGTGTAAGGAATCAATCAACAGAGACGAAAATGTAGTAATTAATTATAACCATTAAGAGGAAGTCGGGAAACCAAAGAAATCTAACATTAAGTCTTTGAAGAACACAAAGCATAATCAAGCATAGAGAACAACATGGCAAAATCATCAAAATCAGAATCACTGATCTCCAGGAAGTGTCTTGATGATGTCGGAATCACCAGGATCAACGATGCTGAGGCAAGAAACTCGGAAGTATTTACCACAAGCAGTACCCAAATCAACATTGTTGCCATTGTAGCGATGAACTCCAACTTTAGCAAGCATCGCATAGTATTCAATCTCTGACCTTCTCAACGGTGGGCAATTGCTAGATATCAATATCAGCTTACCT

>AT3G22420

GTGTCTCTAACACCATCACCAAAAAAGAGACTGTGTTGCTGCCGTGACTCAGTTCCATGTGCCTCCTCTTTCACTTTCTCACAACCCGTGACACTTAACAACTATCACTACACGACAAAAAACCAATCCACAAGTGTTTCGATAAACCTACGACTTTTTTTGTTTTCGCCTTTTGGCATTTCCTTCGGTTATCTCCGTACCGGTTTTGGAATAACCCGGTTCTTGGAATTTAGCTTATCCAAAAACAATCACAGCTTCATAACAACGCATAGCTTATTTATGTGGTCTTAAATCCACTCTTTAGATTCTTTGTTCACACCTGTCAAGTTGTATAATATGCACAATTTTTCAAACATTCTTTCTTCAAAATTCCACAAATATTAGTTGACCACAAAAATTCAATAGAAGAATTTTGGTATAGTTCGTACAGATGGAAGTTGAAATTAGTTGAATTGCCGTTTTCTATACGTACGAATTTTGGTATTTATTGATTAGATAAACCGGAACAAAACCGGTTTAGTAAACCGACGCATGGTGTTGGTAAAGTGTGATTGGGTAAGAAGCCTAACTAGTTGTCTGTTGATAATTGTCACTAGGTGGGCACTTAAAGAAAAAAACACTTGTTTCCTTATATATTCTTCTCCCTTTAAACATTTAATCTTTTCCTCTTCTACCATCTCCACAAATTCCAAACATCTCTCTCTCTTTCTCTCTCACACACAAAATTGCAGAAGAAGAAGAGTCATGAATGGTGAAGAAAGCTTTGTAGAAGATTGCTCTGTTTTTGTTGAGATTGATCCTTCTGGAAGATATGGAAGAGTAATGTTTCTTCTCAATCAAAAGATTTTGACTTTTGGTTTACCTAAAACATGATTTTGATTTGTTGTTGTTTGTGTAGTACGATGAAATACTTGGCAAAGGAGCTTCAAAGACAGTGTAATTGCATTCATCTTCTATCTTTCTCTTTCTTTAAAAATTTACACTAAACAAAAATAATCATTTTTTATTGTTTAATTAGTTTTTTTGGTTACTTTGTTGGTTTGGAATTGGTTACTTAATAATCCAAACACTGTTCTTTGGTTTTATGAAATTTCAGATACAGAGCATTTGATGAGTATGAAGGTATAGAAGTAGCATGGAACCAAGTAAAGCTTCGAAATTTCACAAGGAATCCTGAGGAATTAGAGAAGTTTTTCAGAGAGATTCATCTTCTCAAGACTTTGAATCATCAAAACATTATGAAATTCTACACTTCTTGGGTTGATACCAACAATTTATCAATCAATTTTGTCACTGAACTCTTCACCTCTGGTACTCTCAGACAGTAAGCAAATTCAATCTTATGTCTCTATTACTTCTTTATGAACTCATGGGTACTTGAAAGTTCAAATCTTTTTCGATTTTTAGTTTTTTGCTCTGTTGTTTTGTTAGGTATAGGTTGAGACATAGAAGAGTGAATATTAGAGCAGTGAAGCAATGGTGCAAGCAGATTTTAAAAGGGCTTCTTTATTTACATAGTCGTTCTCCACCAATTATACATAGAGATCTCAAATGTGATAACATTTTCATCAATGGAAACCAAGGTGAAGTCAAGATCGGTGACCTTGGACTCGCTGCGATTCTTCGTAAATCACATGCCGTTCGTTGCGTTGGTACTTCAAAACCTTCACATCATTGGAACTTTATTGCTTTAATTATGTTTTTTACAACTTTGGACCTACCTTTACTATGTTTATGTGTTGTTAAAGGAACCCCTGAGTTTATGGCTCCAGAAGTGTATGATGAGGAATATAATGAGTTGGTTGATGTATATGCTTTTGGCATGTGTGTGTTGGAGATGGTTACTTTTGATTATCCTTACAGTGAATGTACTCACCCGGCACAAATCTACAAGAAAGTTACCTCGGTAAATTATCTTGGACTTGCTTGTTCTTGAAGCTAATTTGAGGTCAAGTCTTGTTTTACTTACATTCTTCATTTCAGGGGAAAAAGCCTGAAGCTTTTTACTTAGTGAAGGATCCTGAGGTTCGTGAGTTTGTTGAGAAGTGTTTAGCTAACGTGACGTGTAGGCTAACGGCATTGGAGCTTTTACAAGACCCTTTTCTACAAGATGATAATATGGATGGATTTGTTATGAGACCTATTGATTACTACAATGGTTATGATGAAACTGGTGTGTTCCTTAGACATCCTTTGATTGATGATCCTCTTTACCATGATCAGTTTGAGTCGTCACAGATATGTGAGATCGATCTTTTCGCTAACGATGATGAAGATCATGTCGACATTTCGATTAAAGGGAAGAGAAACGGTGATGATGGGATATTCTTGAGACTTAGAATATCTGATGCTGAAGGTATAGTTTCAATCTTTTTCGATAGTTTTAAAATCATCGGTCTTAAAAACGTAACACGCTTTCGAGTTTTGGGTAATGTAGGACGGATAAGGAACATTTACTTCCCGTTTGAGACGGCTATTGATACTGCATGGAGTGTAGCGGTTGAGATGGTGTCAGAGCTCGACATAACGAATCAAGATGTTGCGAAAATCGCGGAGATGATCGATGCAGAGATTGCTGCATTGGTGCCTGATTGGAAAAATGATACAGAAAGTTCCCAAAATGTAAACAACAACAAGAACAACAACACTGCAGGATTCTGTGGAGAGTGTGCTTCAAACGGGTATATACAAGAGACTGTATCATCAGGAGAAAAATCTCATCATAATCATCATGAGTTCGATAGTTCTGAAGACAAGAGCTGTTCTTCGGTTCACGGTAGGTTTGCGGATATGTGGGGTTTGCGAGAATCATATTCTGATGATGGAGAAAAACAGAGCTCAAGGAAGGTTAGAAGTGGACGGTGGTCGGAGAATGAGATGAGACGAGAACTGAGATGGCTTAAGGCAAGGCACAAGATTCAACTTATGAAAATGAGAGGTCAAACGATCTGCGAGACACCGATAGAGATCTCTCTTACACCGGGAACTTCAGTTTCGTTACCTCTTCTTTACAGGGCTATATCACTTCCTGTGGATGCCGTGGATATGTGACATTATTGTAAAGTCTGATGATAGTTATCACTTATATTTGTATGTTTCGACATTTTTAACTTTTGTTAAGTTAAAAGTTAATGACATAGTGTACTTTATTTAAAGAGAAGAAGAGTAAACGAAAAACATAAATTAGGCATTGTAAATTGTGGACTAATCATCTAGGTTTTTTCCTTGATGGCACATATCTCCCATAGACTACATATGTCTCTTCATGTGCAATAAGTCTAGATATGTTTTCATCATCACATCGACGTTTTTCTAGACTGCTTTCTTGTTTCCTTTGGCATCCTGGAGGTTGCTTGATTTGCACTAAACTTGGAACATAAGAGCACACAAATAGTGGGGAAAAGTGTTTGTCAACATCAACAGGTTGTCCTAGATGCAGTTCTTTAACGTTTCCAGCCTCTCCCACGATGTAAACTGTCTCGCGATTCATCGGTATATCAAAAATCATAGCAACTTTCTTTACCTCGTCAATGAAAAAACTCCCTTCCAGAAGGTCAACATACATATTCACTGTCAAGAACTTGCTCCACGAAATCTTGTCAGTCTCAATCTTAGTCGTAATCCATACCTCAACAGTAGCATTGTTACAAAACAAAACCGCAAGCTTCTCTTCTCTAACACAAGATAGAGTCACAAGGCTTCC

>AT3G48260

TGAGATATAAGTAGAAGTAATAGATTATAACAAATGATCATAACATTAGTGTAGTGATTTGTGTCAGTACAAGTTTCTATTTAAAGTGTAACAACATTGAATATTTATGTTTCACCAAAAACTATGACTATTATTCTATTAATCCTGAGAGAGAGAGCAAAACCCTTTAGTGAAAAGTTTCCTTAAGTTTAAAAGAACTGAAGCTGAAGTCCAAATTGTTGTTTACACACAGTAAAATCGAATTCGTTACCATGACAAGCGGGTTCAATGGCTAAACATTTTGTAATTTACCAAACCCATTATTCGATTTGTAGGATAGTCAATTATAGGGTTCTTAGTCAGCTAAAACAGTGACTTAAGAGTTAAGAAGGGGAAAAAAAAAGTCCGAATATTTTATATTCACAACACCATGAAGTTGACTGAATAAGCAAAATAATTGATTAGAGTAACATGACAATAAATGAGAAGAAAATCAAATAAAAAACCAGATCCATGGCGCGAAAAAAGCAACGACCCACACCTTTGCCACGTGGTCTTGTCCAGTGGATCTGAGTGTCCTAAATTCGAAGATGAGAAAGAGAGAGAGTGTACAACATTCTACAGTCGACACCATTATGATCAGCAGCTGGAAAAGCTTAAGCTTGGTTTTAATGGCACAAAATCTAAATAATCGATTTATACACAAAAAGACAGCTAAGTAATGATTATTATTATTATCAGAAACTGCTTCAAAACTTGTTCTTCCTTGACTAAATTTCTCTCTTCTCTCAAGAACTCCTATCATCTGTTTTGAATTGCAAGTAACCAAGAATGCGACAAGACGAGAATAACTCCGAGGAAGAATTCGTTGAGATTGATCCCACTGGTCGCTATGGCCGGGTCTGTTCTTCTTTACTTGATTTTAGTGTTACCTTTTTCTCAATTCTAATGTTTTTGTTTCTCATGTTTTCAGTATAAAGAAGTTCTAGGCAAAGGAGCTTTCAAAGAAGTGTATCCTTTTTCTTAGATCTCTGTTATTACTACAGAGACAAATCAGATTAGATTCGGACAAGACACAGTCACTAAAGGGTTTCTTTGATCAGAGAGTTCTTCAGATACAGAGCATTTGACCAACTTGAAGGAATCGAAGTGGCTTGGAACCAAGTTAAGTTAGATGATAAATTCTGTAGCTCAGAGGATTTAGATCGTCTTTACTCTGAAGTTCACTTACTCAAAACCCTTAAACACAAAAGCATCATCAAATTCTACACTTCTTGGATCGATCACCAACACATGACTATCAATCTCATCACCGAAGTCTTCACTTCCGGAAATCTTAGACAGTCAGATTCCTCTCTCAGAAGTCAAATCAAATCTTTTTTATTTGATCAATATTATTCATGTTTGACAAACCCTTATAGGTACCGGAAGAAACATAAGTGTGTTGATCTAAGAGCATTGAAGAAATGGTCAAGGCAGATCTTAGAAGGGCTTGTTTATCTACATAGCCATGATCCTCCTGTGATCCATAGAGATCTTAAATGTGATAACATTTTCATTAATGGGAACCAAGGTGAGGTCAAAATTGGAGATCTAGGGCTAGCCGCGATTCTGCACCGCGCTCGCTCTGCTCATAGCGTCATCGGTGAGATCTTTAATCTTGTTCTCAAACGAAAAAGTTTCTCCATTGTGAAACTATAATTTTGTGTTTGAAATTGTTTAAGGAACTCCTGAATTTATGGCGCCGGAACTCTATGAAGAGGACTATAATGTACTTGTCGATATCTACGCGTTTGGAATGTGTTTGCTTGAGCTGGTAACTTTTGAATACCCGTATTCCGAATGCACAAATGCTGCTCAGATTTATAGGAAAGTTACATCGGTAAGTGATCATATTCCCATCTCATATCCAATCAAGAATGACTTTTTCCATATTGTCGATCCATTTTTCTCAATGCTTCTTAGGGAATCAAACCAGCCGCTCTTTTAAATGTGACTGATCCGCAAGTGAGAGCATTCATAGAGAAGTGTATCGCAAAGGTCTCGCAACGCTTGTCAGCCAAGGAACTACTTGATGATCCTTTTCTGAAATGCTACAAGGAAAATACCGAAAACGTCAGCTCTCACAAAGGTAATATCAGATTATGGTATTTCATTTCATCTCAATATGAAGTGTCATGGACTCCTTTCTTAAAAGTTAAAATCAATAATAGTAACATAAGATAGTTTCTATATTTTTTCTATGTGAAATTTTCTAACACCAAGTTCAATAACATTGTCACAGAAAATGGTTACAACGGAAACGGAATCGTGGATAAACTCTCGGATTCTGAGGTAGGCTTATTAACCGTAGAAGGCCAACGTAAAGACCTCAACACAATCTTCCTAAAACTACGTATCACCGATTCCAAAGGTTCAAAATCTTTTTTGCTTTTTTTATTACAAATCTTCAGATCATTACTAAATCTTCTTTTGACACAATAATAACATTTTTTATACAGGTCAAATCCGCAATATCCACTTCCCATTTAACATAGAGACAGACACATCTTTCTCAGTCGCCATCGAAATGGTCGAAGAACTAGACTTAACCGATGATCAAGACATCTCAACGATAGCTAAAATGATCGACACAGAGATACATTCACACATCCCCGATTGGACCCCTTCTCGACTTATCGGTGATGATTCAGCGGTACAAAAATGTTTGTCTTCTCCAGAGACGCTCCATTTAGATAGATTTCCTTCAGGGAGAAAATTCTGGTCCTCTCCTAAAGCTGGAGCTGGAGACTCACGTTCACCGTTTGCGCCACGGTCTAATTCTAAGTTGTCGTCAGCACAAGGACCGATCAATCAAGAAGTTGGGGTTATAGTAGAGAAACTTGAGTCTTTGTTGAGGAAACAGAGAGAGGAGATTGAAGAGATGCAGAGAGATCAAGAACGTATTGTTACTGAGTTCTTAAAAGAGTTTCCTCCTGAGATTTGTGAAGAGGCTTTGGTGAGATTGCAAGTTAAAGATTCTGATAACTTATTGTGTTAGTACGTTTTTTACATTACACACTCTCGTCAAGATTAATTTGGTTTGCAATATTATGTTGTACTAAAGGTAAGTAAATGTGTAACAAAATTGTGACAGTTATGATTGAAGGAAAACTATTTGTCAAATAATATTTCTTGAATTAGTTAGGTGAAGACGTAACTAGCTCAGAAGAGTCTGGGAGAAGAAAAGAACGCGGTGATGATAGGTCACATCACTGTGTTCTTATTCACTTGATTCTTCTGAGCTAGCCTCGTCCTCTTCACCCAACTCTTATGAACCTTACTCGTCTTCACTGGACTCACTTGCAGAGACTCTAATAAGGTTGTCTTATGATCTATGCTCGCCTCTCATAATCTTAGAAGAAGATATAGAGCGGTTCATATTTTGTTCTCTCTCGCCTATTAGGTTTAATGGGTACTGGTGTTTCTTAAATAGCGTATGCCTCAAGATAATATATTAGGGTTTCATAGATAATAAACCTAATCACCAAAACATTTGGCATAAATGGGCTAAAAGCCCAAAATTTCATAAAACTACCTTAAAACATCTTTATAGGAAAGAAAGCGTTTCAGGGAGTAAGCAAATATAAGCATATGGCAGCGTCAAGGACTCAAGGGATCCAATCAAAACTACAAAGAGCAAATAAATAATCAAAACAAAAACTGAAATAAACTAAATTCTCATACTTGTTC

>AT3G51630

AGTAAAGAGTTTTAAAAAAAAATAAATAACAGCTTTTAATATGGCTAATGCGTTCAGTTTTTGTGATTGGTAACTATCAAAATGGTAAGGTCCAATTGTTGACATCAAATCGTAAGATTTTTTTAGGGTGAGCAGAAGTATAATATCGCTATTTTTTCCGCTTTTGTGATTGGTAACAATCAAAATGATAAATCATTCATTGTGAAAATCATCACTGAATTCCTTATGTTTTGTTTTGGTCTATGTATAGATTTAGTTGAACCCAATGTGACAAGTTTATTATTCTCTCAATCAGTGCTGGATTCTGCAAATCACTTTCAAAAACATTGAATTCGAATATTGTAATAAGAAAAACAAAAATTGTGGGGAGGGGAAATAGTCAAATAAAAGGGTAATGGTGTCAAAGTCACGCAATCATCGGTTACATACATTACTCTTACATATCGACATACTTAACGTTAATCAAATCTCGTTTGACAAACCATCATCAATAAAGAGCACAAGAACAAAGAAGAAGGAAAAAGTAGAGCTTTTTTGGGAATCAGAATCATCATCGATGGTGAATCCAAAGCGTGGCAATAGGTTTTTCAAATACAGAATTGTGTAAATTGCTCTCTATCTTCTTCTCTTTCAAATTCTCTTCCTTTTGTTGTATTCAATAACTTTTTCCCCACTTTGATGGGCTGCAACCACATATTCTTCTTCTATCTTTAACTTTTCCCACCAAACTTCTCCTTTTTTTTCTTTTTTTTTGGGTTCTGGGTCTGCTTTTTTGTTGTTGTTGTTGTTGAATGAAGGTCCGTAAGGGAAGAAGACAGATGATTGCGAAAGAGCAAGACGAATACAAATTACGCCAACAAGGTCTTGTTTTTCAAATGGCTTCTCTTTTCCTCTCTTTCGATTACTCCTTACTCTTCTTTTGGTTGCTCTTCCGCGTTTTCTTATACGAGAATCACGAACGAAAACTCCAGATTTTCTAGCACACCTCTTCTCTCTGCATCGCCATCTTGTTTTTTCCCCCAAATTTTCGATTTTTTTTTTGTATAACTTTTGAGATTCTGATAGATCCGGTACGGAGTCATGTATATGGAGATTTCTTCTGCTTCCGATGATTCGATCGCTTACGTTGAGACAGATCCTTCTGGTCGCTACGGACGTGTAAGCTTACTCTCTTTCTCTTAGCTGATTGCTATTTTGATCAATCCCCAAATTTGATTACTTTTTGGCTCTCTCTCTTTCGATTATCTTTTGCATAACTTTGGCACTATCTCTTCCATTGGTGTTGTTCTTTGGCTTGGTAATATACTTGCTTCTAGTTGCTGGACATTTGCTTTGATCGTAGCTTCTCCTCTCTTTGCATTTTCTAGAGTTGATTTGTTTTTGATTACTGGGTTTGCTCTCGAACTTTGGGTTGATTTCAGTTCAGAGAAGTACTTGGCAAAGGGGCGATGAAGACGGTTTATAAAGCATTTGACCAGGTTTTAGGAATGGAAGTTGCTTGGAATCAAGTTAAGCTCAATGAGGTTTTCCGATCACCTGAGCCTTTACAACGCCTCTACTCTGAGGTTCATCTCCTCAAGAATCTCAATCACGAATCCATTATTCGTTACTGCACGTCTTGGATCGATGTCAACCGCAGAACATTCAACTTCATTACTGAGCTGTTTACATCAGGCACCCTTAGAGAGTAATGAGAATCCCCTACAGTGTGTGTTAAACAACAATATAATCTTATGCTTGTGCAGAATTCCTTAAAGAGAGAAGCTTGTTGTGTGTGTCTTACATCTGCAGGTATAGAAGGAAGTATCAAAAGGTTGATATCCGGGCAATCAAGAGCTGGGCACGTCAGATCTTGAATGGTCTTGCTTATCTCCATGGACATGATCCTCCTGTCATTCACAGAGACCTCAAATGCGATAATATCTTCGTTAATGGACACCTTGGGCAAGTTAAGATTGGTGACCTGGGCTTAGCTGCAATTCTTCGTGGATCACAGAATGCGCACAGCGTCATAGGTATTCTATGTCTTTCTCTATGTTTTTGAATAGAGTTTTGTGAGTTCCATAATTAATCTTTGCCTTTTGCTTGCATATACCATTAGGAACGCCTGAATTTATGGCACCAGAGCTATATGAAGAAGATTACAACGAGCTTGTAGACATTTATTCGTTTGGTATGTGTGTCCTAGAGATGCTTACCGGTGAGTATCCTTATAGTGAATGCACCAACCCTGCCCAAATATACAAGAAAGTTACATCGGTAAGCTAATGGCCTGAGGCTCATATCAATCATCATTTTCTTCTCAGAAAATATTTTGATATATTAACTTCTTTGATGCGGATTTGATTCAGGGGAAGTTGCCTGACTCATTCCATCTAATCCAACACACGGAGGCCCAACGTTTTGTTGGTAAATGCTTGGAAACTGTGTCAAGAAGATTGCCTGCAAAGGAGCTCTTAGCGGACCCATTCCTTGCCGCAACTGATGAGAGAGATTTGGCGCCTCTTTTTAGATTGCCGCAACAATTGGCAATCCAGAATTTGGCTGCAAATGGAACGGTGGTAGAGCATCTGCCGTCAACGACTGATCCAACTAGAACAACAGATATGTCAATAACAGGAAAGATGAACTCAGAAGACCACACCATCTTCCTTCAAGTACAGATTCTAGATGGCGATGGTAAGTTAAAGTTCTACTTACTACAAATGTCTTGCCTGGTTTGATTGGAACATGAAATTTGTTGCGTTGCTAGAAACTTGCTCAGCTGAACCGGGCTACAGGATTTGTAGCACCTTTAGTATTAGCTATTCATCATAAGATAGAAATGGACGATTGTAACCTTAGCTTCTGATTTTTGTAAAACTTTGTTAGAAGAATCACCAATGGAAAATGTTTTTATGAATCTGTTTGGCTGTGGCACAGGTCACATGAGGAACATTCAGTTCCCGTTCAACATTTTAAGTGACACACCTCTAGAAGTAGCTTTGGAGATGGTGAAGGAGCTTGAGATCACTGATTGGGATCCTTTGGAGATCGCTGCAATGATAGAAAATGAGATATCTTTGCTCGTCCCCAACTGGAGGGCCAATGACTCTTCTATCCGGCACGAAAGCTTTGGTCATGAAGATGATGAGGACAATGGCGACACAGAGGGAAGAACGCGCCTTTTCTCCTCTGCTTCTTCCTCTCACGACTCTCCTGTAGCAGTTAGAGAGAACAATGACGATTCTAGTAACGATGTGATCCCAGATATGGATGATGGCAATAGAAGCTCTAACAGGTTGCTGAATTCTTCAACATACCACTACTCACCTGCCATTGATGATGATCAAAATCAGCAACAACGGAGACGAGTAAGGCTACAACAGAAAATGAGATCGCTGGTGGACACGAGAACACAGGTGCTACACCGATCGCTCATGGAGTTGATCAACAAGCGTCGTGGGCGTGGCTTTGACCCGAACACAAACGAGCTACAACCTCAACCGTCGTCCACTGATTTCATTCGACGATGTTGAAAATAGTATGATGAGAGAGCCTATTTGTGACTCTTTCATGATAAAGACAGATCTTCATATTGGTACTAGCATTTTGATTTGATGATACTATATGAAATAATTGTTTAGAGTGCTTTGTATATATGATATTCTTAGGGAAGTGTTACCTTTTTAGCTCTATCTCGATTTGAAAGCGATGCAAAACCGCTCGAATTCGATTTCAAGTACGAAGGTAGCTTCTTCCCTGTGTTAAAGAGACAATTTAGATTACATTTTATGCATTTTCCATTACAGTGGTAACTTAATATTCAAATAATCATAGGAATTAAGGATTTTTCTAATCTTGATACGAAAGTTGTTTATCTTCTCGTATCAAACGTAGAGGCAGTTGAACAAATTTACAAACGAAAATGTTCTTAGATTGGTATAACCATTCGTCGAAAACTATAAAGATTTACTTTTATTTAACCATAAGATGATATCATCTATCAAAAAGTGTTAATTATAGTTGATCATATATAATAAGAACAAATTATAAAGGCTATATACAAAGGAAATTTGCGTTAATTAGGTTATCTAACCGCATGCATCCAAAACAAATCATGTATTTAAAAACTTGATCACAACTTGAGCAAAATATATGGAGAGCAAAGGGCCATCGCCAATAGTTCCACTTGTGCTTGTGGTACTCTTCGGATGGTTCCTATGCGGCTCGTACCTTGTCTCACTATTAGAAGATGTTGTATTACCAATCTTACAAACCGGAACGTTCATTGTAGGAATCTTTCTC

>AT5G28080

AAGATTTGGAAAAAAAAAACTGAAGCAATGATATTTAGTGATTTGCGTAAACTAAAATTTATGTGGCACAATAAAATTGAATAAATAATTATATATGACTTTTAAGTGATTCACTCATATATATTTTACAATTAAGGTACATATAAATTTGCTCGTTAAAAATATTACAATTATATAGTGTGTATACATGAACATAATATCTTAAATAATATTGAACAAGAAAATATCTTTAAAAAGCACCACGCGTCTAGTTAGAAACTAATAAGATAAGAAGTTTATTTCCTATATCTTTGATTTGATATAAAGATAATATCTAAGAAAAATGTATCTTAATCAAACACTTGTTTGTTGCGAAAAAGAAACAAAAAAGATTTGTGGATGTCAATAAAAAAAATCAAAGATTTGTTTATTCGTATCATAGTATGAACCACGCTCATTTCGTCTTGCACCCACCTTTATTTACATCTTTCATGAGATATAATAGTCATGTTTCAGTGTAAGAACGACTAAAGAAAATTCTTTCTCCAAAAAAACAACAACTAAAGAAGCAATAAAAAAACAGAGAAAAAAAGGAATCTAGTTTTTTTGTCTCCTCATTTACACTGAGTTTAATGTTTAATTTGTTTAACTTATTATGTCAGAAGTACAAAGATGATTATGATGAAAAGGCTATAAGAAACCCATAACATAGATATTTAAATATATTTGCTTAGCTCTTTTCATTTTTTGGGACTTCTTGGTTTCTTTATATTTTCCAGCTATTGTATTGCTCTTTCTGATCTTTGTTGGGGTGTATGATGAACAATCTCAGCCATCTTGAATCAGATTACTCTGAGTATGTTGAAGTTGATCCTACTGGAAGATATGGAAGAGTAAATTTCCTTTATATCTCTCAATAATTTGTTTGTTTATTATGATCATCTCTTTTGTTTTGTTGAGTTTTTTTTGTTATATGTGTGTGTTCTTGTTGTTTTAGTACAATGAAGTTCTGGGTAAAGGATCTTCAAAGACTGTGTAAGAATCCAATTTCTCTCTTAAGTTTTCTTACCTTTTTTTCAAGTATCAACTTCTCTAAAACTTTGGTGTTGGAGTTTCATGCTTTTCAAAGACAAGTTCTTACTTTTTGTTTCAAATTTTCTTAGTAATTTTGATTATTTTTCATTTTCATAGATGTTTTTTTTTTTGTATTTTTTTGAGTTTTAGCTTTTTACTTTTAGTGGTGGTTGATTCATGATTGATTTTTAAAAAGATTTATCATTTCAAAGCGAAAAAGGTTGAAATCTTTGATCATTAAACAAAAAATGGGTTTAGTGATTCTAAACTAGAAAAAGACTATGTTTTTGGTTCCATAATTTGGATTTGCAGTTACAGAGGATTTGATGAGTATCAAGGTATAGAAGTAGCATGGAACCAAGTAAAGCTCTACGATTTCTTGCAGAGTCCTCAAGAGCTAGAGAGGCTCTACTGTGAGATCCATCTCCTCAAAACCTTAAAACACAAAAGCATCATGAAGTTCTACGCTTCTTGGGTCGATACCGATAATCGAAACATCAACTTTGTCACTGAAATGTTCACTTCTGGCACCTTGAGACAGTAAGCTTATCTCTCTATCTCATAAGTGAAGTTCTCTTGATAACTTACTCATAATGTTTTTATATCTTTCATGCAGGTATAGGCTAAAACATAAGAGAGTGAACATAAGAGCGGTAAAGAATTGGTGTAGACAGATTTTAAGAGGATTAAACTATCTTCATACACATGACCCTCCTGTGATTCATAGAGATCTCAAGTGTGACAACATTTTTATAAATGGGAACCAAGGAGAAGTCAAGATTGGAGATCTTGGTCTTGCTGCTTGTTTACAACACTCTCACGCTGCTCATTGTGTTGGTAAGCAATCTCTCTTCAGTGTTTTTGCTTTGCTGAATCCATCAATAGTTGGATTATGATCTGTTGAAAGTTGTTTGATATCTTTAGGCACACCAGAGTTCATGGCTCCTGAAGTTTATAAAGAAGAATATAACCAATTAGTCGATATTTACTCGTTCGGTATGTGTGTTTTGGAAATGGTAACGTTTGATTACCCGTATAGTGAGTGTTCTCACCCTGCTCAGATTTACAAGAGAGTTATATCGGTAAGGATCCATCCGTGTTTACCGCGATTATAACATCTCTTATGTTGAGTTTTAAGATTGTTAAAGTTATCTTTCTCAGGGCAAGAAACCAGATGGATTAGACAAAGTGAAGGATCCAGAGGTTAGAGGGTTTATCGAGAAGTGTTTGGCCACAGTGTCCCTTAGACTCTCTGCTTGTGAACTACTAGATGACCATTTTCTTTGTATCGATGAATCGGATATGAGACGCGTAGAGAGCGAAAAGGGCTTGATAGATGAAGCAGGGACTCCTCTTAGACATTCTTATCATATACCTCACTACTCAAACGGTTACTACAGCCTCTATAATCAAAACCAATGGGATTACAATGGAGATGAGACAGTGGAGTCACACGAGATAGATCTCTTGGAGTTTCAAAATGATGATGATGAAGAAGAAGAAGATAAGAGGTTTGGTAGTGTAGATATAAGCATTAAAGGGAAGAGAAGAGACAATGGAGATGGACTATTTCTGCGACTCAAAACCGTCAACAAGGAAGGTTGTGTGAGAAACATATACTTCCCGTTCGACATCGAGACTGACACAGCAATAAGCGTTGCAAGAGAGATGGTGGAAGAGCTGGAGATGGATGACCGTGATGTCACAAAGATAGCTAATATGATCGATGGAGAGATTGCTTCTTTGGTTCCTAATTGGAGTATCTTCTGCAGCTCCGAATCGAACCGTTCCTCGGTGGGTTCAGTCATGGATTTCAATGAGATGCAGTGTGGAAGAGACGGGTGTGAAGAGAAGCATGGACGGTTTGAGGAGATTACGTTTGAGATAACAGTAAACGACTCTGACGAAGAAGACTAGTTTTCTTGTGCGGCAAGTAATGTGATACACTAGCTATATGGATCTAAAACCTTTCTCTTTGTCCATAGTCTTATGTTAACACTATTGATCATTGGGAATGTGGTTCACAAACAGATGAATAATTCCATTACTAACCGTTAGCATCTGAATCACACCATGCCAAAGGGAGAAGAGAATGTTGTAATCTTATTAATCTTAAAGAGAGCGTATATATACATGGTACAACCGTACAAGAAGCCCTAAACACACTTAAGATATTAACAAAATAGTATAGGTAATATAATACATTTACTTATAACCCGCTAATAGTACCGAATCTCTCCTCGTCACCTCTGCATTTACTAAGAATGTGCATTGCGAGCAACATGTTACAGTTGATATTATCTTGAACAAAATACCTTTGGTAGCCAAATGAGTCTCCATGCTTCAGTTATTAGGGTCCATAAGCTTTGTTTATAGTAAAAAAAATAGGAAAAGAACGTCACAAACTACTATGAAGGAATTTTTCGTTTAGAATTGAAGCCACAAAGACATACAAAACATAACGTTATTTGAAACGTCATAGGGCTTTAAGCTTTGAGTAAAACATTTGCTGAAGATTTGTATGGGTAAGTCCTAATTAAAGACTCTTTACATTTTTATAATACTAAATTTTGAATAATTTAAAGTTTAGAGTTTTTGTTGAATCCTTATATTTTTTTATTGTTTAATGGTG

>AT5G41990

AATATAAGATGTTATAAAAGTTTATCTTTTAAATATTAAAAAATATTTTGTTAGTTTCTATGAAAATTAAATGTATTTGATAATTTGTGATCATTTATATTCTGCAGTTTTTTCTTATGATTAGTTAAAATTTTTAATTCTATAAATAATATTTTTTAGAAAGAATGATCTTAATATGTGTGATTGTTTTAAACATTTTATATTTTGAAACAAAGGAAATAACATCGTATTTCTGAGGATTTACATAATGTTTTTTTTTTGGTGCACCTAGCATAATGTTTTATTAGTAGAAATTATTCATAACCTGAAACATGTAATTGCATAAATGGCCTAAAGTCAGCTGAAATAAAATGAAAACAGAGGATACATAATTAGTAGAATAATCTCAAATATAAGAGAAAGTGCGTTAGTTGATAAAGACAGATAATTTGACTTTCCGATTTATTTGGCCGCCAACCTTTTTTTGTTTTGTTCTTCCTAAATTCCCTTTCTCCGAAATTCCTTTCATTGATTTCATCATCATCATCATCCTTCGTTTTTTCTCTATCGATCTAGCAGATTCTTTCGGGGACCAAAATCAAAATCATGGTGGATCATCAATGGAAGGATTTAATCGGATAAAAGAGAAGAGACGGAATCACGACGGGAGAAGAGATCGGGAAATCGGAAAATCGGAGATGATGGGGATTTCTTTCGCCGCCAAACTCCGTTTCCGATCTCGATTTCGAACTTCTTCAATCGATTCTTATTGCTTCGCTCGTGAGGCTTTCTCCGATTGTATCTCCTCCGTCCATTTCTTCTTCTTATAACCTTTTTCTTTGTAATAACCTCCGTCCTCTTCAGCTTTCTTTCTTTTCATCTTCAATCTCACCTTAAATTCTCCACTTTTTTCTTCTTCTCCTTCTGTTCTCGATTGCTTTGTTTGTTGTGTTGTGCATACATATATGGCTTCTGGTTCTGGATTTTTAGGTCAGATATCGTCCATGGAAGAGGCTGATTTTGCCGAGAAAGATCCTTCTGGCCGTTACATCAGGGTACGTCACTGTCTTCGTTGATTCATGTGTGAATTTGCATTCAATCTCGTTTGGTTGAGTGTTTTTTATTGTTGAATTATGAGGAAATTGATCAGAATTTGAGAATTTTGACCTAGTTTAATGAGTTCTCTTTGTAGTTCTACCTAATGAGGGCTGTTACTTTGATCGTATGGTGATGATTATTGGTTTATAGCAATGTGTGTTTTGTTCATTGTTACGTGTTTTCGACTTTTAAGCTATGTACAATTGTACATTTGGAACAATCAAGCTTAAGATCATGTTGTATTGCTGATTTTTTCGAGTTTGTATGGTGGAAAACATATAGTGATTGATCGATTCGTTTTTTTTTTGTTGTTGTTGTTTAGTATGATGATGTTCTGGGGAGAGGAGCTTTCAAGACTGTGTATGTTTTCTACACCCTATTATGCTTTTATGTTTTTAGTTGTAAAGGAAAGTGATTCTGATTTGTTTATTAACTCTTGTATAGATATAAGGCGTTTGACGAAGTAGACGGGATTGAAGTTGCTTGGAACCTAGTTAGCATTGAAGATGTAATGCAGATGCCTGGTCAACTTGAAAGGCTATATTCTGAAGTTCATCTACTAAAGGCTCTAAAACATGAAAACATCATCAAACTCTTTTACTCTTGGGTTGACGAAAAGAACAAGACTATTAACATGATCACTGAGCTTTTCACCTCTGGTAGTCTCAGGGTGTAAGTATCTCCCCCACTTCTCTTTTGTATTGATGATCCTTCATTCTTTTTTTTTTTCCTGTGCATCACATGGGTTGACACCATTTCATTTGATGTGAAGATATCGTAAGAAGCATAGGAAGGTTGATCCCAAGGCCATCAAGAACTGGGCAAGGCAGATTCTCAAAGGCCTGAACTATTTGCATTCTCAGAACCCTCCGGTAATTCACCGGGATCTGAAGTGTGACAATATATTTGTCAATGGAAATACTGGAGAGGTCAAAATCGGAGATCTCGGGCTCGCAACTGTACTGCAGCAGCCCACTGCTCGAAGTGTGATAGGTAATACACCATAAACCATTTTCCTTCCTAGACCCTGAAAACTTTACTGTAACTGAAAACTTTGAGCGTAATCAGGTACTCCTGAATTCATGGCACCTGAACTGTATGAAGAGGAATACAATGAGCTTGTGGACATCTATTCTTTTGGCATGTGTATGTTGGAGATGGTAACCTGTGAATATCCATACAACGAGTGCAGAAACCAGGCTCAAATTTATAAAAAGGTCACCTCGGTAAGTCTTTCACAATCTTTACTTTCATCAATTATCTATGCAATGGGGCTCCCGTTTGAAACTTTGCATTTGCAGAACATAAAGCCTCAGTCTCTTGGCAAAGTTGACGATCCTCAAGTTAGGCAATTTATAGAGAAATGTCTTCTTCCTGCCTCTTCCAGACCAACTGCCCTCGAGCTCTCGAAGGACCCATTCCTTGCAAGGGATGGAGGCAAGGACTCTGCTCTGCTTGCTTCATCAAGCACATCATCTAAATATGTGAGACCACCACAGCTTGAACATCTTCCCATGGATGTGGATCATAACGAGAATAAAAGTGTTTCAAGCAACGAAGACTACCCATGGTCCCAAACCATTGAACTTCAGAGGATTGCAGAGAATAAAGAGTTCAGGTTGAGAGGTGAGAGGAGCGATGATGTCACAGCGTCTATGGTTCTCCGTATTGCTGACCCGTCTGGTTCGTCTTATAAAATTAAATCTTCTATCCAATTCATTCCTCTCACAGTTTTCATTTACTGAAAATCAATTCAATGCCCTAATACAGGTAAATGTAGAATCGTACACTTTGCATTCTATCTGGAGTCAGACACAGCAACAGCAATCGCTGAGGAAATGGTTGAGGAACTGCATTTAACTAGCCAAGAGGTCGTTGTGATAGCTGATATGATCGATGACTTCATAATGCAACTTCTCTCTGACCGGACCTCGTCGCATCATAACCAAAACTCTCCACGTCTAACCCATGAGGATCATGAAGCAGCAAATCAGCAAACTGTGAACTCGAAAGATGAAGAAGCTGCAGGACAATCAATGAAGTCAGACATATCAGCAGACTATTACTTTCCCTACTCAGCAAACGACGGAAATGCTGCCATGGAAGCTGGTCGAGATGCAGAGTCGATGAGCTCGTATCTGGATTCTTGCTCAATGATGTCAACCATTTACAATCTTTCCATTTCAGACAATGATTACCCAGAAGATCTCAAGACAGAACTAAACCTGATCGAGTCACAGTTTAACCAGTCCTTCCAAGATCTGCTGAAACTGAAAGAGGATGCAATAGAGAACGCTAAGCGAAAATGGATTACGAAAAAGCAAAAAGCAGTTAACATCTCTTGATTCAAAAGCTCTGTTTTTGATATTTTACTCTTCTTTTGGTCTTCAACTTTGCTGAGATCGGTGTGTTATACATTTGGTGTTTTGGATATATATATTTGATTTGATTTGATACAACAAACATAAATAGTGGTATTTGACTTATATCATTTCTAACAGACTAAACACACGAAACCTGTTAAAAAAAAACTAAAAAGTTTAGAAGGTTGACATATTTTGGACATAATGGTTCCAAAGCTCAGATGCAATCTCATCTCTTAACCTTAAACTATCTTCAACTTCTTCTGGTCTAAACCCATGTTCCTGCCCCACTATCTCTACTTGTTCCTCTTCTAAAGCCACTTCTCTATCTTCTCCTGCTTCCGCCAGAGTCTCTTCTTCAAACATCCTAAACACTAAATCATCTGGTTTCTCCAAGCGGACATAGTTGTGCAATGCACAAGCCGCAATCACCAGTTTCACTTGCGTTTGTAGAGGATACGGAGGAGCTGATAGTAGAATCGGAAACCGTTCTTTCAAAGCCCCAAAAGTCCGGTGGATAGCTCGATGCAACAACTTGTGTCGCTCATTGAACATTTCCTTTGCTTCTTCTCTTGAATTGGTTGAAACACCGTGATACGG

>AT5G55560

TATTCTTGCTCCTCCAATTGATAAAAAAACGACGGCCTCGATTGTTTTACTTCTTCACGCGCCGAAAGTTTGACTTTTACTTATGTAAAGTCCACGAAATATATTAATTACATTCTTATACTAAATAACTATAAGATTATGCGGTTAAGTTCTGACACAAAATTTAACCCAAAAAGTTTTTTTTTTCTTTTTTGTTTTTTTTCGAAAAAGCCCAAAAGAAAAGTTTTAAAACACAAAATGACCAAATCTATTTCAATTAATTTTACAATTTAACTTGTAAATTTTATTTTATCCCATGTTAAACTGGTAATAATAATAACTCAAATAATATGGTATCAAATATTATTATTATGTTAATAATTTGCCTTAAAAAATCGGTGTCAACGATTTGGTAAAAGCCTTGAAGAGTCCAATTCCAGATAGGCATGGAAAAACAGAGGAAAAAAAGAAAGAATATTTACTGGTATATATATATATTAAAATCACACACACAATCTCTTCCGCTTCTTTGGTCTTTAAAACCTTTCCCTAAATCTATCACGCTCCGTTACGTTTTTCTCCTTTACGAAAATCTCAGCAAAGAATTTTCGATAATTCTCTCCTCCGATTCAAGCGATTTTTAATGGTATGTGTTCTTCAATCTCTTTATGTGTGTGTGTATATGTGTTATGTTCTAGTTGGAGTTTTTTTTTTTCTTCTTACGTTTATTTAGGGTTCTTATATACATGTTACCGATCTAGTTTTTTTTTAAATTAGTTATCAATCTTCAATTTGATTCTAGTACATGGAAAACTGAATTTCAAACTGGTTTTATCTGAAATCTTCTTTGTTGTTGTGAAATTCGTGTAATCAGAATTTTGAGTCTGTTTTACTAGCAGATGACTTGTGCGAGCTCCGATGATAATGAGAGTGAAAAGGACAAGGACTCGGAGTCCTTCGTGGAGGTAGATCCGACAGGTAGATACGGTCGGTACGGCGAACTTCTTGGCTCAGGCGCCGTAAAAAAAGTCTACAGAGCCTTTGACCAAGAAGAAGGCATCGAGGTGGCTTGGAACCAAGTCAAACTGAGATGTTTTTCGGATGATCCAGCCATGACCGAGAGGCTTTACTCGGAGGTCAGGTTGCTTAAGAACCTTAAGAACAGTAACATCATCACCCTGTATAAAGTGTGGAGAGACGAGAGAAACAACACCTTGAACTTCATCACCGAGATCTGTACCTCCGGAAACCTGAGAGAGTACCGGAAGAAGCACAGACACGTCTCTATGAGGGCTTTGAAGAAGTGGTCCAAACAGATTCTCAAGGGCTTGGATTATCTCCACACTCACGACCCTTGCATCATCCACAGAGACCTCAACTGTAGCAATATCTTCGTCAATGGCAACATCGGCCAGGTATTTTACTCAATCGTAAATTGTTCTACTAGTGTGTGTGTGTGATTGAGTTGAGTTGTGTCTTGATGATTGAAAATGATCAGGTCAAGATTGGTGATCTAGGTTTAGCTGCAATTGTGGGGAAGAACCATTTAGCTCACTCGATCCTCGGGACACCAGAGTTCATGGCTCCTGAGCTGTACGAGGAGAACTACACTGAGATGGTTGACATATACTCGTATGGAATGTGCGTTTTGGAGCTTGTGTCACTCGAGATTCCTTATAGCGAATGTGACAGCGTCGCCAAAATATACAAAAGGGTGAGCAAAGGCCTCAAACCAGAGGCTCTCAACAAAGTTAATGATCCAGAAGCTAAGGCATTTATTGAAAAATGCATCGCGCAGCCGAGAGCTAGACCTTCTGCAGCTGAGCTCCTCTGCGACCCGTTCTTTGATGGGATATTAGATGATGATGACGAAGACGGTGAAAACAATGACAACAATGGAGCTGGTCGTATTGTTGTTTCTTGATGAGTGTATACAATGAACCTTGGTTAATTAGATGTTTGGTTATCAACTGCACGCTCCATTTCTTCCAAGCCTGCAGTTGTTAGTAAAAGTTATATAGTAATGTTTTTCTCTAATAGCTTTTGACTATAACAGAAGAAGATGGACTCTAAAATCGAATCAACTTACGTAGCTTACCGAAACTGTAAACATCTGACTTGACCAAGTATGTCCCCTCTTTCGAGTATTCCGGAGACATGTAGCCACTGCATATTTAAATAACACGATTAAACAAGACGTTACTTGGTTTCAGGTCCCTATGTACAATGATTAGAGTTGCCGAATATTCGTGAAGTCCACGTGCAACACCTATGTCAATTTCAAATCTCTTGGGCCTATTTAGTTTATCACTTTGGGTTTCGTCTACAGATAGATTAATTTAGTTTATAACTCTGGATCAGATTAGTTTCCTTGTCCATATAATATCAATTTGGTATTGGGCTGCGTCTTCTTCTCTTGAAAAAAAAAAGAAAGTAAACAATCAATATTCATGGTCTTGCTAGACTAATCTTAATTGTTTTTTTTTTCTTTCTTTCTTTAACTTTGTTCTGTAAATTATATACATTTACCAACCTATAATTAGCAAGGAAATAACTCCACACACAAAACCCTCCAAATCGCCAAATTTTGAACTTTTAATTACCTTCCACAAAAAATGCAGATTAATCATAAAAAGTCAAATTAATATGCAATAAAACCACTCTTCAAATGTATATTGTATCATA

>AT5G58350

CTGTAGAGTTATTTACCTTTGTGTATTGTGATATATCAACTGTGGAGAACATGGTGGAGAAAGGGCCATTCCCATGTTGTGTGGTGAATGATAATGATGAATGAGCAGAATATAATTATATGCTTGTGGGAAAAATTCTGGCTGCAACCTCTGTAGTCAGTCAGTGTCAAACTTATACGGCTTTTCTTGTTTTAGATATATTCCATCCTTATCTTCATTTCCTAATTCCTACCATGCTTCCCTCTCTCCTTTTCCACGTGTCAACTAACATCTAAACCAGAACAAAACAGTAGTCAAAAAATGCATAACCATTTTGATCTATGATATTGCCTGTCAAACATTCGGACCCTCCTTAGATATTGCCTGTTAACATTCAGACCCTCCTTAGATAAAAAGCGAAGTAATATGATGGGAGCTCTCATGCACATACTTTATTGGTGAATGCATATTTGTTACGTGTTATTAGTTTATTACAGAACCGTGAACTCCTTGAGGGCTGCAGGAACTGGCCGCCAACAAAAACAAAACAATTTTCAACGGTGATGTCACTATCACTCTATTCTCTACAACTTCTTGTCATGTTCCCCACTTCTGTTCTGACTTTTCCCCTTTTCTTTGTCGTTTTATTTTTGTTAACCTCAATCTATTTATTTTGGATTGCTTGTGTTCACCAAACTAATAATTGGTTCAATTGAAAATTGTGCAGTTTCCTCAAGAAACAAAACTCTTTCCTGAATATCGCATTTCCCAGTATCCACACTTTCTTAACTCTTATCCTCTTTGCAATTGTGTTTGTGGTTCACAAAATAAACAAGGGCATCTTTTGAGCATATTTAGCCATGTGTTTTCAATGTGGTACACGTTTGATGATTTTTTTTTCATTTTTGTGGTTTGAGTATCTTGTATTGGCAAGTAAGATTTGTCAAACTTTCGATTTTTAGATTTACAATTGGGTCAATAAAAATAAATATATGATTTTCTATAATATATGTGATGCAAGCAAGGTTAAGGCAAATTGTCTTTATTCTTTTTATCCCAAATGGGTGATTGACGCCAACGACTGTTCAACATATTTTCTGCTGACGCTTCTTTAATGAAGTAGCACGAAGAAGTAAACAGGACGACTTTTGGCCACCGGAAAATTTCTCTGTTATAAAAACGAAAGTCTGTGTCCTCTTAAACATTTTTGGGATCATCAATCGGTTTTTGGTCAACCATGAATATGAATCAAGTTGCAGAGTATGTGGAAACTGATCCAACTGGTCGCTATGGACGTGTAAGTAAGGAGACAGATTCTATGTTCATGGATCAAAACCTTGTTGTAACACTTGGTTTTTTATGTTGGAACAGTTTGCAGAAATTCTTGGAAGGGGAGCGATGAAGACAGTGTACAAAGCAATCGACGAGAAGCTGGGAATAGAAGTAGCATGGAGCCAGGTGAAGCTTAAGGAGGTTCTGCGTTCCTCTGTTGATCTACAGAGGCTTTACTCCGAGGTTCATCTCCTCAGCACTCTCAACCACAAATCCATCATTCGTTTCTACACTTCCTGGATCGATGTTCATAACCACACTCTCAACTTCATCACCGAGTTGTTCACTTCAGGGACCCTCCGACAGTAATTCGCTTAAACCTCTTCTTCTGACAAGATTATTCAGCTTTTGTCTTTTATGACCCTGTCCCGTTCATATGCAGATACAAAAATAAGTACTTGCGGATAGATATCCGAGCAATCAAGTCCTGGGCTCGGCAAATCTTGGAAGGGCTAGTTTATCTCCACGAGCACGACCCTCCTGTTATCCATAGAGACCTTAAGTGTGATAATATCTTTGTGAATGGGCATCTTGGACAAGTCAAAATTGGTGATCTTGGTCTTGCAAGAATGCTGCGGGACTGCCACTCGGCCCATAGCATCATTGGTATGTCTCAAAAATCGTTTGTTTTCACTTCTTGTCCAAATCATATGCTTAGACCTCTTTGTGTTTCTTTTGCATAGGCACTCCTGAGTTCATGGCACCAGAATTATATGAGGAGAACTATAATGAACTCATTGACGTATATTCCTTTGGTATGTGCTTTTTGGAGATGATCACCTCTGAGTTCCCGTATAGTGAGTGCAACCATCCGGCACAGATTTACAAGAAAGTTGTTGGGGTATGTCTTACAATCTGTGAGCATATAAATGAAGCTGTCAGATGAACCAGGCATGAGTCTTCTAATGTTCTCTTTTCAGGGAAAGCTACCAGGAGCATTTTACAGAGTTGGAGACATCGAGGCACAGAGGTTCATCGGGAAATGCCTCGTGTCTGCCTCAAAGAGAGTATCAGCAAAAGAACTATTGCAAGATCCATTTCTTGCCTCCGATGAGTCCTGGATGGTATACACGAGCGGTGCTGGGAATCCAAAGCCCTTCTTGAACGAGAATGAAATGGACACACTGAAGTTGGAAGATGATGAGTTAAGGACCGAGATGTCCATCGCTGGGAAGCTGGGCGCTGAAGATAACAAAATCGATCTGGAAGTACAAATCGCATATGATAATGGTAAATGGGACTAAATTGATTAAGCTGGTCACTTTGTTATTCGGTCGCATAATTGTTTTTTTCTTTCCAGGTCTGGCCAATAATGTATTCTTTCCCTTTGACATCATGAATGATACTTCCATTGATGTTGCAAAGGAGATGGTAAAAGAACTCGAAATTATAGATTGGGAGCCAGTCGAGATTGCTAAAATGATTGATGGAGCGATTTCTTCTTTGGTGTCTGATTGGAAGTATGAGGAAGATGATGAAACCCCTCATGATCATCATCGTCATCGTACCGATTCTTTTCACTCTTCTTCCTCGCACGCGTCATCTTCCCAAGCTTCACTCTCGAACTATATGGCTCGAGGCCTTCAAGATTGGGTACAAGGTATGGAAAGTAACACCAGTCTCAACGTGCATAGTGTGACAATTGTTTTTATAAAAGTTTTTGAGATAGAAATTAAAATTGCAGATGATTTACACGATGAGACGTATTCTCAGAGTTCTTCCCATTCAGGGTCTTACTCCAACCTCAACTACATAGCTGTTGATGAGTACAGCTCCCAATCTCCTGTTATGAGCAGAACTCACAACATGACGAGGTTTTGCCCCGAAGAAAGCTCTCATCTACAGTCAGGACAAGCCAACGCGTATGCAGCTTCCAGTTCAACTAACAGAAGCTTGGCTTCAGACAACCGCACACTTACAAGGAACCGATCACTTGTAGACGTGCAGAGACAGTTACTGCACCGGTCGCCAGGGGAAGAGGCTAGAAAAAGAAGATTGTTCAAAACTGTTGGAGACGTAGAAACAGTTGGGTTTCAGTCACCTTATGCGGTTTCACGGAAGCCACCAAGCTCAAGGCGCTAGCTTCTCCAGTATTTTAATTACTGTAATATATAAGTATACTGTAAAAAACAAGTTTGCTGGTTCAAAAATGTAAAAAGTACATGTGTTTAATAAAGTTGTTCAAAGGGTGTGTTTGTGTTACAGAGAAAGAGTCCTCATGGTGAAATGACTCAAATGTAACCATCTATACCAGAACAAACAGTAGTCAAATATAACCATTTTATCTATGCATGATATAGCCTGTTAAAACTCGGATCCTCCTTAGATACAAAGCTAAGTAAGATGATGAGAGCTCTCATGCACATATATATATGAAATGGTTGATGCCATATTTGTTGTCTTATTATTAGATATCTTACAAAAACATATGTGTTATGTTTTAATGACAATTTAGCTGACATAGAAGGATAGTTTAAAGTTTCTAGGTACGGTCTTGTCAATGCAAGTGATAGATGAGCTTCAAGTAGATGCATTGTAATCCAAGCCACGGAGAAACGCCATGTAGTCTGATTTGCCTAATTCAAAGTCAAGTAAAGCTGCTATTGGGTCGACATGTCCAGCTCCATAGTAGAGTATTTATTAGTTGGGTTTGGCTTTATCCACAAAAGCTCATTTTAAACCAGAACGATCTAGCATTTTGG

Example command to run SMAP design with option --preSelectedPrimers.

python3 SMAPdesign.py HOM04D000265_ath_selected_genes_extended_500_bp.fasta HOM04D000265_ath_selected_genes_extended_500_bp.gff -g Ath_FlashFry.tsv -v -smy -o SMAPdesign_with_psp -gl -al -psp HOM04D000265_ath_preSelectedPrimers.gff

Examples of files generated as output with option --preSelectedPrimers.

Summary plot

contig	start	stop	target	context	overflow	orientation	Doench2014OnTarget	DoenchCFD_maxOT	DoenchCFD_specificityscore	Hsu2013	otCount
AT3G22420	0	23	TTACTAAACCGGTTTTGTTCCGG	NONE	OK	RVS	NA	0.4750337382537112	0.2650838869375759	9.528.446.540.584.890	19
AT5G41990	0	23	ATGATGATGAAATCAATGAAAGG	NONE	OK	RVS	NA	0.3535714284892857	0.20242156026472147	9.393.208.757.483.550	40
AT5G58350	9	32	AATTGTTTTGTTTTTGTTGGCGG	GTTGAAAATTGTTTTGTTTTTGTTGGCGGCCAGTT	OK	RVS	0.10016007087579634	0.6500000002499999	0.051030487720633504	7.596.095.569.737.420	137
AT3G48260	10	33	CGACCCACACCTTTGCCACGTGG	AAGCAACGACCCACACCTTTGCCACGTGGTCTTGT	OK	FWD	0.6873256423101545	0.0	1.0	100.0	1
AT3G22420	11	34	CATGCGTCGGTTTACTAAACCGG	CAACACCATGCGTCGGTTTACTAAACCGGTTTTGT	OK	RVS	0.1912165422108699	0.4326923076140734	0.5711470610715759	996.960.519.344.634	9
AT5G58350	12	35	GAAAATTGTTTTGTTTTTGTTGG	ACCGTTGAAAATTGTTTTGTTTTTGTTGGCGGCCA	OK	RVS	0.012535624621428638	0.807692307625	0.03952364449929724	6.483.151.877.417.350	176
AT1G49160	12	35	ATAGTTTGGAGAGATAAATCAGG	ATATAAATAGTTTGGAGAGATAAATCAGGTGGAAC	OK	FWD	0.03994653264470359	0.5090497736114252	0.43245399487926967	9.716.930.139.370.150	15
AT3G22420	12	35	CGGTTTAGTAAACCGACGCATGG	CAAAACCGGTTTAGTAAACCGACGCATGGTGTTGG	OK	FWD	0.42843723377477166	0.028758169734640522	0.9720457435180724	9.998.281.191.993.750	2
AT3G04910	12	35	GAAGTATGTAAAAAGGTTTAAGG	TATTTAGAAGTATGTAAAAAGGTTTAAGGAACAAA	OK	FWD	0.021268209504280614	0.35117056868478264	0.28100326272596843	9.327.845.772.956.530	17
AT3G48260	13	36	AGACCACGTGGCAAAGGTGTGGG	TGGACAAGACCACGTGGCAAAGGTGTGGGTCGTTG	OK	RVS	0.5737596036901703	0.08484848503636364	0.8950019370545259	9.990.337.925.076.550	3
AT3G48260	14	37	AAGACCACGTGGCAAAGGTGTGG	CTGGACAAGACCACGTGGCAAAGGTGTGGGTCGTT	OK	RVS	0.07355588977432755	0.17982017953796203	0.7724169389198978	9.941.400.784.077.560	4

Gene	Total # amplicons	Total # gRNAs	Amps with gRNAs	Amps with 2 gRNAs	# gRNAs after TTTT filtering	# gRNAs after restriction site filtering	# gRNAs after intron filtering	# gRNAs after complete filtering
AT1G49160	2	265	2	2	210	208	92	51
AT1G64630	2	305	2	2	276	269	99	61
AT3G04910	2	306	2	2	261	256	141	93
AT3G18750	2	302	2	2	246	244	106	65
AT3G22420	2	231	2	2	193	191	108	57
AT3G48260	2	225	2	2	194	190	88	55
AT3G51630	2	302	2	2	266	263	126	85
AT5G28080	2	192	2	2	166	164	75	44
AT5G41990	2	283	2	2	248	247	104	71
AT5G55560	2	182	2	2	160	154	80	49
AT5G58350	2	316	2	2	276	271	137	87
average	2	264.5	2	2	226.9	223.4	105.1	65

AT1G49160	Araport11	gene	501	3639	.	+	.	ID=AT1G49160;symbol=WNK7;tid=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	mRNA	501	3639	.	+	.	ID=AT1G49160.4;Parent=AT1G49160;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2899	3639	.	+	.	ID=AT1G49160.4:exon:1;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	three_prime_UTR	3420	3639	.	+	.	ID=AT1G49160.4:three_prime_UTR;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2899	3419	.	+	2	ID=AT1G49160.4:CDS;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2339	2759	.	+	.	ID=AT1G49160.4:exon:2;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2339	2759	.	+	0	ID=AT1G49160.4:CDS;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2066	2223	.	+	.	ID=AT1G49160.4:exon:3;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2066	2223	.	+	2	ID=AT1G49160.4:CDS;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	1749	1969	.	+	.	ID=AT1G49160.4:exon:4;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	1777	1969	.	+	0	ID=AT1G49160.4:CDS;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	five_prime_UTR	1749	1776	.	+	.	ID=AT1G49160.4:five_prime_UTR;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	989	1648	.	+	.	ID=AT1G49160.4:exon:5;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	five_prime_UTR	989	1648	.	+	.	ID=AT1G49160.4:five_prime_UTR;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	501	719	.	+	.	ID=AT1G49160.4:exon:6;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	five_prime_UTR	501	719	.	+	.	ID=AT1G49160.4:five_prime_UTR;Parent=AT1G49160.4;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	mRNA	382	3639	.	+	.	ID=AT1G49160.2;Parent=AT1G49160;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2899	3639	.	+	.	ID=AT1G49160.2:exon:1;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	three_prime_UTR	3420	3639	.	+	.	ID=AT1G49160.2:three_prime_UTR;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2899	3419	.	+	2	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2339	2759	.	+	.	ID=AT1G49160.2:exon:2;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2339	2759	.	+	0	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2066	2223	.	+	.	ID=AT1G49160.2:exon:3;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2066	2223	.	+	2	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	1749	1969	.	+	.	ID=AT1G49160.2:exon:4;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	1749	1969	.	+	1	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	1421	1648	.	+	.	ID=AT1G49160.2:exon:5;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	1421	1648	.	+	1	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	1242	1279	.	+	.	ID=AT1G49160.2:exon:6;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	1242	1279	.	+	0	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	989	1111	.	+	.	ID=AT1G49160.2:exon:7;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	1025	1111	.	+	0	ID=AT1G49160.2:CDS;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	five_prime_UTR	989	1024	.	+	.	ID=AT1G49160.2:five_prime_UTR;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	382	719	.	+	.	ID=AT1G49160.2:exon:8;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	five_prime_UTR	382	719	.	+	.	ID=AT1G49160.2:five_prime_UTR;Parent=AT1G49160.2;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	mRNA	501	3639	.	+	.	ID=AT1G49160.1;Parent=AT1G49160;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2899	3639	.	+	.	ID=AT1G49160.1:exon:1;Parent=AT1G49160.1;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	three_prime_UTR	3420	3639	.	+	.	ID=AT1G49160.1:three_prime_UTR;Parent=AT1G49160.1;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	CDS	2899	3419	.	+	2	ID=AT1G49160.1:CDS;Parent=AT1G49160.1;Name=AT1G49160;gene_id=AT1G49160
AT1G49160	Araport11	exon	2339	2759	.	+	.	ID=AT1G49160.1:exon:2;Parent=AT1G49160.1;Name=AT1G49160;gene_id=AT1G49160

AT1G49160	Primer3	Primer_forward	1707	1728	.	+	.	ID=AT1G49160_Amplicon01_fwd;Sequence=TGGAGTTGTGTTTAAAGCAGCG
AT1G49160	Primer3	Primer_reverse	1830	1849	.	-	.	ID=AT1G49160_Amplicon01_rev;Sequence=TCGGCGGATCTTGACTATGT
AT1G49160	Primer3	Primer_forward	2449	2473	.	+	.	ID=AT1G49160_Amplicon02_fwd;Sequence=GGATTCTTTTCTCAATGTGAACGGT
AT1G49160	Primer3	Primer_reverse	2569	2590	.	-	.	ID=AT1G49160_Amplicon02_rev;Sequence=TGACATCGTTCTATTCCGAGCA
AT1G64630	Primer3	Primer_forward	2391	2414	.	+	.	ID=AT1G64630_Amplicon01_fwd;Sequence=AATACAAGTGTTTCCATCTGCTCT
AT1G64630	Primer3	Primer_reverse	2491	2510	.	-	.	ID=AT1G64630_Amplicon01_rev;Sequence=TCTCCTCTCTCCACTCAGCT
AT1G64630	Primer3	Primer_forward	2218	2237	.	+	.	ID=AT1G64630_Amplicon02_fwd;Sequence=AGTGTCTTCTCCCAGCTCCT
AT1G64630	Primer3	Primer_reverse	2331	2350	.	-	.	ID=AT1G64630_Amplicon02_rev;Sequence=GGTGGCATTGCAGGTTTGAA
AT3G04910	Primer3	Primer_forward	3439	3458	.	+	.	ID=AT3G04910_Amplicon01_fwd;Sequence=CCAATCCTGGGGCAAATGTG
AT3G04910	Primer3	Primer_reverse	3556	3575	.	-	.	ID=AT3G04910_Amplicon01_rev;Sequence=GCTTTCTTGCTGTTGCTGCA
AT3G04910	Primer3	Primer_forward	2037	2058	.	+	.	ID=AT3G04910_Amplicon02_fwd;Sequence=GTGCAGACAAATCTTGAGAGGC
AT3G04910	Primer3	Primer_reverse	2164	2185	.	-	.	ID=AT3G04910_Amplicon02_rev;Sequence=TTCTTAAAATAGCAGCAAGGCC
AT3G18750	Primer3	Primer_forward	3256	3275	.	+	.	ID=AT3G18750_Amplicon01_fwd;Sequence=CGTCACGTTTATAGCCGAGC
AT3G18750	Primer3	Primer_reverse	3382	3402	.	-	.	ID=AT3G18750_Amplicon01_rev;Sequence=TGTTTCTGTGGCTTTGCTTCG
AT3G18750	Primer3	Primer_forward	2874	2894	.	+	.	ID=AT3G18750_Amplicon02_fwd;Sequence=CGGCCTAGTAAAACCTTGTCG
AT3G18750	Primer3	Primer_reverse	2985	3006	.	-	.	ID=AT3G18750_Amplicon02_rev;Sequence=CCTTGAGGACAAAGAAATTCCC
AT3G22420	Primer3	Primer_forward	1594	1613	.	+	.	ID=AT3G22420_Amplicon01_fwd;Sequence=GATCGGTGACCTTGGACTCG
AT3G22420	Primer3	Primer_reverse	1713	1739	.	-	.	ID=AT3G22420_Amplicon01_rev;Sequence=CACATAAACATAGTAAAGGTAGGTCCA
AT3G22420	Primer3	Primer_forward	2406	2425	.	+	.	ID=AT3G22420_Amplicon02_fwd;Sequence=CGTAACACGCTTTCGAGTTT
AT3G22420	Primer3	Primer_reverse	2528	2551	.	-	.	ID=AT3G22420_Amplicon02_rev;Sequence=CGATTTTCGCAACATCTTGATTCG
AT3G48260	Primer3	Primer_forward	2571	2593	.	+	.	ID=AT3G48260_Amplicon01_fwd;Sequence=CCGATGATCAAGACATCTCAACG
AT3G48260	Primer3	Primer_reverse	2690	2709	.	-	.	ID=AT3G48260_Amplicon01_rev;Sequence=TGGAGCGTCTCTGGAGAAGA
AT3G48260	Primer3	Primer_forward	1908	1933	.	+	.	ID=AT3G48260_Amplicon02_fwd;Sequence=TCCATATTGTCGATCCATTTTTCTCA
AT3G48260	Primer3	Primer_reverse	2020	2038	.	-	.	ID=AT3G48260_Amplicon02_rev;Sequence=CTGACAAGCGTTGCGAGAC
AT3G51630	Primer3	Primer_forward	3022	3041	.	+	.	ID=AT3G51630_Amplicon01_fwd;Sequence=ATCCTTTGGAGATCGCTGCA
AT3G51630	Primer3	Primer_reverse	3138	3157	.	-	.	ID=AT3G51630_Amplicon01_rev;Sequence=CTTCCCTCTGTGTCGCCATT
AT3G51630	Primer3	Primer_forward	2389	2408	.	+	.	ID=AT3G51630_Amplicon02_fwd;Sequence=CGGAGGCCCAACGTTTTGTT
AT3G51630	Primer3	Primer_reverse	2517	2536	.	-	.	ID=AT3G51630_Amplicon02_rev;Sequence=TGGATTGCCAATTGTTGCGG
AT5G28080	Primer3	Primer_forward	2346	2366	.	+	.	ID=AT5G28080_Amplicon01_fwd;Sequence=TGAATCGGATATGAGACGCGT
AT5G28080	Primer3	Primer_reverse	2468	2489	.	-	.	ID=AT5G28080_Amplicon01_rev;Sequence=TCTCCATTGTAATCCCATTGGT
AT5G28080	Primer3	Primer_forward	2514	2539	.	+	.	ID=AT5G28080_Amplicon02_fwd;Sequence=AGATCTCTTGGAGTTTCAAAATGATG
AT5G28080	Primer3	Primer_reverse	2638	2658	.	-	.	ID=AT5G28080_Amplicon02_rev;Sequence=ACAACCTTCCTTGTTGACGGT
AT5G41990	Primer3	Primer_forward	2609	2634	.	+	.	ID=AT5G41990_Amplicon01_fwd;Sequence=CGAGAATAAAAGTGTTTCAAGCAACG
AT5G41990	Primer3	Primer_reverse	2732	2754	.	-	.	ID=AT5G41990_Amplicon01_rev;Sequence=GGTCAGCAATACGGAGAACCATA
AT5G41990	Primer3	Primer_forward	2906	2925	.	+	.	ID=AT5G41990_Amplicon02_fwd;Sequence=CGCTGAGGAAATGGTTGAGG
AT5G41990	Primer3	Primer_reverse	3031	3051	.	-	.	ID=AT5G41990_Amplicon02_rev;Sequence=CCTCATGGGTTAGACGTGGAG
AT5G55560	Primer3	Primer_forward	1177	1199	.	+	.	ID=AT5G55560_Amplicon01_fwd;Sequence=ACGAGAGAAACAACACCTTGAAC
AT5G55560	Primer3	Primer_reverse	1298	1319	.	-	.	ID=AT5G55560_Amplicon01_rev;Sequence=GAGATAATCCAAGCCCTTGAGA
AT5G55560	Primer3	Primer_forward	1501	1520	.	+	.	ID=AT5G55560_Amplicon02_fwd;Sequence=AGCTGCAATTGTGGGGAAGA
AT5G55560	Primer3	Primer_reverse	1620	1639	.	-	.	ID=AT5G55560_Amplicon02_rev;Sequence=CACAAGCTCCAAAACGCACA

AT5G58350	AT5G58350_Amplicon01:gRNA01	CTCTCTTTGAGGCAGACACGAGG
AT5G58350	AT5G58350_Amplicon01:gRNA02	TCGAGGCACAGAGGTTCATCGGG
AT5G58350	AT5G58350_Amplicon02:gRNA01	AGGACCGAGATGTCCATCGCTGG
AT5G58350	AT5G58350_Amplicon02:gRNA02	TGAAATGGACACACTGAAGTTGG
AT3G48260	AT3G48260_Amplicon01:gRNA01	GTCACATTTAAAAGAGCGGCTGG
AT3G48260	AT3G48260_Amplicon01:gRNA02	TCTATGAATGCTCTCACTTGCGG
AT3G48260	AT3G48260_Amplicon02:gRNA01	TACATTCACACATCCCCGATTGG
AT3G48260	AT3G48260_Amplicon02:gRNA02	ATCACCGATAAGTCGAGAAGGGG
AT3G51630	AT3G51630_Amplicon01:gRNA01	GCCTGCAAAGGAGCTCTTAGCGG
AT3G51630	AT3G51630_Amplicon01:gRNA02	CATCAGTTGCGGCAAGGAATGGG
AT3G51630	AT3G51630_Amplicon02:gRNA01	GAGTCATTGGCCCTCCAGTTGGG
AT3G51630	AT3G51630_Amplicon02:gRNA02	TCTATCCGGCACGAAAGCTTTGG
AT3G04910	AT3G04910_Amplicon01:gRNA01	CGTTAACGGGAATCAAGGAGAGG
AT3G04910	AT3G04910_Amplicon01:gRNA02	ATGATCCTCCTGTGATCCACAGG
AT3G04910	AT3G04910_Amplicon02:gRNA01	GATCACGATCAGAGAAACCGAGG
AT3G04910	AT3G04910_Amplicon02:gRNA02	GGTGCGGTGAGACTCATGGTCGG
AT3G18750	AT3G18750_Amplicon01:gRNA01	CGCTAAAAGTAACAATAGGCAGG
AT3G18750	AT3G18750_Amplicon01:gRNA02	TTCAGGATCCAGGTGTATTGAGG
AT3G18750	AT3G18750_Amplicon02:gRNA01	TTGTCAACATGATACCCACCTGG
AT3G18750	AT3G18750_Amplicon02:gRNA02	GAGATGATCGACTGTGACATCGG
AT5G55560	AT5G55560_Amplicon01:gRNA01	GCACAGACACGTCTCTATGAGGG
AT5G55560	AT5G55560_Amplicon01:gRNA02	CCGGTACTCTCTCAGGTTTCCGG
AT5G55560	AT5G55560_Amplicon02:gRNA01	CCATGAACTCTGGTGTCCCGAGG
AT5G55560	AT5G55560_Amplicon02:gRNA02	TAGTTCTCCTCGTACAGCTCAGG
AT1G64630	AT1G64630_Amplicon01:gRNA01	GACCAACTCCTTGCAGTAGATGG
AT1G64630	AT1G64630_Amplicon01:gRNA02	AGCAGTAAGAGTGGAGTCCTTGG
AT1G64630	AT1G64630_Amplicon02:gRNA01	AGCAACCCTCTGGACTTCCATGG
AT1G64630	AT1G64630_Amplicon02:gRNA02	TCTGGAGCAATGCGCATTCTTGG
AT3G22420	AT3G22420_Amplicon01:gRNA01	TGAAGTACCAACGCAACGAACGG
AT3G22420	AT3G22420_Amplicon01:gRNA02	ATAAAGTTCCAATGATGTGAAGG
AT3G22420	AT3G22420_Amplicon02:gRNA01	TGATACTGCATGGAGTGTAGCGG
AT3G22420	AT3G22420_Amplicon02:gRNA02	CATTTACTTCCCGTTTGAGACGG
AT5G28080	AT5G28080_Amplicon01:gRNA01	TAGTAACCGTTTGAGTAGTGAGG
AT5G28080	AT5G28080_Amplicon01:gRNA02	ATATGATAAGAATGTCTAAGAGG
AT5G28080	AT5G28080_Amplicon02:gRNA01	GTGTAGATATAAGCATTAAAGGG
AT5G28080	AT5G28080_Amplicon02:gRNA02	AAGAGAAGAGACAATGGAGATGG
AT5G41990	AT5G41990_Amplicon01:gRNA01	AATAAAGAGTTCAGGTTGAGAGG
AT5G41990	AT5G41990_Amplicon01:gRNA02	CCCAAACCATTGAACTTCAGAGG
AT5G41990	AT5G41990_Amplicon02:gRNA01	CAGCTATCACAACGACCTCTTGG
AT5G41990	AT5G41990_Amplicon02:gRNA02	TAATGCAACTTCTCTCTGACCGG

AT5G58350	AT5G58350_Amplicon01_fwd	AGGGAAAGCTACCAGGAGC
AT5G58350	AT5G58350_Amplicon01_rev	ACCATCCAGGACTCATCGG
AT5G58350	AT5G58350_Amplicon02_fwd	CGGTGCTGGGAATCCAAAG
AT5G58350	AT5G58350_Amplicon02_rev	CGATTTGTACTTCCAGATCGATTTT
AT3G48260	AT3G48260_Amplicon01_fwd	CCATATTGTCGATCCATTTTTCTCA
AT3G48260	AT3G48260_Amplicon01_rev	CTGACAAGCGTTGCGAGA
AT3G48260	AT3G48260_Amplicon02_fwd	CGATGATCAAGACATCTCAACG
AT3G48260	AT3G48260_Amplicon02_rev	TGGAGCGTCTCTGGAGAAG
AT3G51630	AT3G51630_Amplicon01_fwd	GGAGGCCCAACGTTTTGTT
AT3G51630	AT3G51630_Amplicon01_rev	TGGATTGCCAATTGTTGCG
AT3G51630	AT3G51630_Amplicon02_fwd	TCCTTTGGAGATCGCTGCA
AT3G51630	AT3G51630_Amplicon02_rev	CTTCCCTCTGTGTCGCCAT
AT3G04910	AT3G04910_Amplicon01_fwd	TGCAGACAAATCTTGAGAGGC
AT3G04910	AT3G04910_Amplicon01_rev	TTCTTAAAATAGCAGCAAGGC
AT3G04910	AT3G04910_Amplicon02_fwd	CAATCCTGGGGCAAATGTG
AT3G04910	AT3G04910_Amplicon02_rev	GCTTTCTTGCTGTTGCTGC
AT3G18750	AT3G18750_Amplicon01_fwd	GGCCTAGTAAAACCTTGTCG
AT3G18750	AT3G18750_Amplicon01_rev	CCTTGAGGACAAAGAAATTCC
AT3G18750	AT3G18750_Amplicon02_fwd	GTCACGTTTATAGCCGAGC
AT3G18750	AT3G18750_Amplicon02_rev	TGTTTCTGTGGCTTTGCTTC
AT5G55560	AT5G55560_Amplicon01_fwd	CGAGAGAAACAACACCTTGAAC
AT5G55560	AT5G55560_Amplicon01_rev	GAGATAATCCAAGCCCTTGAG
AT5G55560	AT5G55560_Amplicon02_fwd	GCTGCAATTGTGGGGAAGA
AT5G55560	AT5G55560_Amplicon02_rev	CACAAGCTCCAAAACGCAC
AT1G64630	AT1G64630_Amplicon01_fwd	GTGTCTTCTCCCAGCTCCT
AT1G64630	AT1G64630_Amplicon01_rev	GGTGGCATTGCAGGTTTGA
AT1G64630	AT1G64630_Amplicon02_fwd	ATACAAGTGTTTCCATCTGCTCT
AT1G64630	AT1G64630_Amplicon02_rev	TCTCCTCTCTCCACTCAGC
AT3G22420	AT3G22420_Amplicon01_fwd	ATCGGTGACCTTGGACTCG
AT3G22420	AT3G22420_Amplicon01_rev	CACATAAACATAGTAAAGGTAGGTCC
AT3G22420	AT3G22420_Amplicon02_fwd	GTAACACGCTTTCGAGTTT
AT3G22420	AT3G22420_Amplicon02_rev	CGATTTTCGCAACATCTTGATTC
AT5G28080	AT5G28080_Amplicon01_fwd	GAATCGGATATGAGACGCGT
AT5G28080	AT5G28080_Amplicon01_rev	TCTCCATTGTAATCCCATTGG
AT5G28080	AT5G28080_Amplicon02_fwd	GATCTCTTGGAGTTTCAAAATGATG
AT5G28080	AT5G28080_Amplicon02_rev	ACAACCTTCCTTGTTGACGG
AT5G41990	AT5G41990_Amplicon01_fwd	GAGAATAAAAGTGTTTCAAGCAACG
AT5G41990	AT5G41990_Amplicon01_rev	GGTCAGCAATACGGAGAACCAT
AT5G41990	AT5G41990_Amplicon02_fwd	GCTGAGGAAATGGTTGAGG
AT5G41990	AT5G41990_Amplicon02_rev	CCTCATGGGTTAGACGTGGA